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Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> RNAi
Overview
Product name
Anti-Dicer antibody
See all Dicer products (11) ...
Description
Rabbit polyclonal to Dicer
Tested applications
ICC/IF, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1200 - 1300 of Human Dicer.
(Peptide available as ab24556.)
Positive control
This antibody gave a positive signal in HEK293 Whole Cell Lysate Overexpressing Dicer
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5818 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/200(Use with paraformaldehyde fixed cells.)
WB: Use a concentration of 2 µg/mlDetects a band of approximately 220 kDa (predicted molecular weight: 217 kDa).Can be blocked with Dicer peptide (ab24556).
Target
Function
Required for formation of the RNA induced silencing complex (RISC). Component of the RISC loading complex (RLC), also known as the micro-RNA (miRNA) loading complex (miRLC), which is composed of DICER1, EIF2C2/AGO2 and TARBP2. Within the RLC/miRLC, DICER1 and TARBP2 are required to process precursor miRNAs (pre-miRNAs) to mature miRNAs and then load them onto EIF2C2/AGO2. EIF2C2/AGO2 bound to the mature miRNA constitutes the minimal RISC and may subsequently dissociate from DICER1 and TARBP2. Also cleaves double-stranded RNA to produce short interfering RNAs (siRNAs) which target the selective destruction of complementary RNAs.
Involvement in disease
Defects in DICER1 are a cause of pleuropulmonary blastoma (PPB) [MIM:601200]. PPB is a rare pediatric tumor of the lung that arises during fetal lung development and is often part of an inherited cancer syndrome. PPBs contain both epithelial and mesenchymal cells. Early in tumorigenesis, cysts form in lung airspaces, and these cysts are lined with benign-appearing epithelium. Mesenchymal cells susceptible to malignant transformation reside within the cyst walls and form a dense 'cambium' layer beneath the epithelial lining. In a subset of patients, overgrowth of the mesenchymal cells produces a sarcoma, a transition that is associated with a poorer prognosis.
Defects in DICER1 are the cause of goiter multinodular type 1 with or without Sertoli-Leydig cell tumors (MNG1) [MIM:138800]. A common disorder characterized by nodular overgrowth of the thyroid gland. Some individuals may also develop Sertoli-Leydig cell tumors, usually of the ovary.
Sequence similarities
Belongs to the helicase family. Dicer subfamily.
Contains 1 Dicer dsRNA-binding fold domain.
Contains 1 DRBM (double-stranded RNA-binding) domain.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain.
Contains 1 PAZ domain.
Contains 2 RNase III domains.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionQ9UPY3
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DCR1 antibody
- Dcr1 homolog (Drosophila) antibody
- Dicer 1 antibody
see all
Database links
- Entrez Gene: 23405 Human
- Entrez Gene: 192119 Mouse
- Omim: 606241 Human
- SwissProt: Q9UPY3 Human
- SwissProt: Q8R418 Mouse
- Unigene: 87889 Human
- Unigene: 21135 Mouse
Anti-Dicer antibody images:
Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody (ab5818)
ab5818 (1/200) staining Dicer in HeLa cells (green). Cells were fixed with paraformaldehyde, permeabilised with 0.5% Triton X100 and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please see Abreview.
Image courtesy of an Abreview submitted by Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
Western blot - Dicer antibody (ab5818)
All lanes : Anti-Dicer antibody (ab5818) at 2 µg/ml
Lane 1 : HeLa Whole Cell Lysate at 10 µg
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate at 10 µg
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate at 10 µg
Lane 4 : U2OS (Human osteosarcoma cell line) Whole Cell Lysate at 10 µg
Lane 5 : HEK293 Whole Cell Lysate Overexpressing Dicer at 5 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 217 kDa
Observed band size : 220 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa,90 kDa. We are unsure as to the identity of these extra bands.
Immunocytochemistry/ Immunofluorescence - Anti-Dicer antibody (ab5818)
ICC/IF image of ab5818 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab5818, 10µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-Dicer antibody (ab5818)
ab5818 has not yet been referenced specifically in any publications.
Publishing research using ab5818? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"