Overview

  • Product name
    Diclofenac sodium salt
  • Description
    Potent cyclo-oxygenase inhibitor
  • Biological description
    Potent non-selective COX inhibitor (IC50 values are 75 and 29 nM at COX-1 and COX-2, respectively). Potent inhibitor of phospholipase A2 in vitro and in vivo. Activates the nitric oxide-cyclic GMP-potassium channel pathway. Shows anti-inflammatory, antipyretic and analgesic effects.
  • Purity
    > 99%

Properties

Applications

Our Abpromise guarantee covers the use of ab120621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration.

Images

  • ab96732 staining Prostaglandin dehydrogenase 1 in MCF7 cells treated with diclofenac sodium salt (ab120621), by ICC/IF. Increase in Prostaglandin dehydrogenase 1 expression correlates with increased concentration of diclofenac sodium salt as described in literature.
    The cells were incubated at 37°C for 6h in media containing different concentrations of ab120621 (diclofenac sodium salt) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab96732 (1 µg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody.

References

This product has been referenced in:
  • León-Reyes MR  et al. Pharmacokinetics and pharmacodynamics of diclofenac in the presence and absence of glibenclamide in the rat. J Pharm Pharm Sci 11:68-76 (2008). Read more (PubMed: 18801308) »
  • Warner TD  et al. Nonsteroid drug selectivities for cyclo-oxygenase-1 rather than cyclo-oxygenase-2 are associated with human gastrointestinal toxicity: a full in vitro analysis. Proc Natl Acad Sci U S A 96:7563-8 (1999). Read more (PubMed: 10377455) »
  • Mäkelä A  et al. Inhibition of serum phospholipase-A2 in acute pancreatitis by pharmacological agents in vitro. Scand J Clin Lab Invest 57:401-7 (1997). Read more (PubMed: 9279965) »
  • Scholer DW  et al. Pharmacology of diclofenac sodium. Am J Med 80:34-8 (1986). Read more (PubMed: 3085490) »

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"

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