Biotin Anti-Digoxigenin antibody [BT.21H8] (ab419)
Key features and details
- Biotin Mouse monoclonal [BT.21H8] to Digoxigenin
- Suitable for: WB, ELISA, IHC-P, IHC-Fr
- Reacts with: Species independent
- Conjugation: Biotin
- Isotype: IgG1
Overview
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Product name
Biotin Anti-Digoxigenin antibody [BT.21H8]
See all Digoxigenin primary antibodies -
Description
Biotin Mouse monoclonal [BT.21H8] to Digoxigenin -
Host species
Mouse -
Conjugation
Biotin -
Tested applications
Suitable for: WB, ELISA, IHC-P, IHC-Frmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Synthetic peptide corresponding to Digoxigenin.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: PBS, 50% Glycerol, 1% BSA -
Concentration information loading...
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Purification notes
Purified biotin conjugate. -
Clonality
Monoclonal -
Clone number
BT.21H8 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab419 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/5000.
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ELISA |
1/1000 - 1/10000.
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IHC-P |
1/200 - 1/2000.
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|
IHC-Fr | (1) |
1/200 - 1/2000.
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Notes |
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WB
1/500 - 1/5000. |
ELISA
1/1000 - 1/10000. |
IHC-P
1/200 - 1/2000. |
IHC-Fr
1/200 - 1/2000. |
Target
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Relevance
Digoxigenin (DIG) is a steroid found exclusively in the flowers and leaves of the plants Digitalis purpurea and Digitalis lanata. Digoxigenin is chemically closely related to Digoxin, the cardiac glycoside used for the treatment of various heart diseases. The term 'genin' at the end of Digoxigenin, refers to only the aglycone portion (without the sugar) part of the molecule,thus Digoxigenin is the steroid component of Digoxin, - minus the (digitose) sugar residues. DIG can be covalently added to proteins or nucleic acids which makes it very useful in diverse applications. -
Alternative names
- 1672-46-4 antibody
- BRN 0096479 antibody
- DIG antibody
see all
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab419 has been referenced in 6 publications.
- Wang P et al. Circular RNA circBNC2 inhibits epithelial cell G2-M arrest to prevent fibrotic maladaptive repair. Nat Commun 13:6502 (2022). PubMed: 36316334
- Zheng F et al. The HIF-1a antisense long non-coding RNA drives a positive feedback loop of HIF-1a mediated transactivation and glycolysis. Nat Commun 12:1341 (2021). PubMed: 33637716
- Jia J et al. Transcriptional factor FoxM1-activated microRNA-335-3p maintains the self-renewal of neural stem cells by inhibiting p53 signaling pathway via Fmr1. Stem Cell Res Ther 12:169 (2021). PubMed: 33691791
- Heine S et al. CNGA3: A Target of Spinal Nitric Oxide/cGMP Signaling and Modulator of Inflammatory Pain Hypersensitivity. J Neurosci 31:11184-92 (2011). IHC-FoFr . PubMed: 21813679
- Silva VA et al. Expression and cellular localization of microRNA-29b and RAX, an activator of the RNA-dependent protein kinase (PKR), in the retina of streptozotocin-induced diabetic rats. Mol Vis 17:2228-40 (2011). In situ hybridization . PubMed: 21897745
- Arendt T et al. Selective cell death of hyperploid neurons in Alzheimer's disease. Am J Pathol 177:15-20 (2010). In situ hybridization . PubMed: 20472889