Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)

Overview

• Product nameAnti-Dishevelled 2 (phospho S143) antibody [EPNCIR145]See all Dishevelled 2 primary antibodies ...
• Description
  Rabbit monoclonal [EPNCIR145] to Dishevelled 2 (phospho S143)
• Specificityab124933 only detects Dishevelled 2 phosphorylated at Serine 143.
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Mouse, Human
  

  Does not react with

  Rat
• Immunogen

  Synthetic phosphopeptide corresponding to residues surrounding Serine 143 of Human Dishevelled 2.

• Positive controlF9 cell lysate
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPNCIR145
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Cytoplasmic

  • Stem Cells
  • Signaling Pathways
  • Notch
  • Cytoplasmic

Applications

Target

• FunctionParticipates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
• Sequence similaritiesBelongs to the DSH family.
  Contains 1 DEP domain.
  Contains 1 DIX domain.
  Contains 1 PDZ (DHR) domain.
• DomainThe DIX domain mediates homooligomerization.
• Cellular localizationCell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.

Target information above from: UniProt accession O14641 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1856 Human
  • Entrez Gene: 13543 Mouse
  • Omim: 602151 Human
  • SwissProt: O14641 Human
  • SwissProt: Q60838 Mouse
  • Unigene: 118640 Human
  • Unigene: 5114 Mouse

• Alternative names
  Dishevelled 2 (homologous to Drosophila dsh) antibodyDishevelled dsh homolog 2 antibodyDishevelled-2 antibody

  Dishevelled2 antibodyDSH homolog 2 antibodyDVL 2 antibodyDvl2 antibodyDVL2_HUMAN antibodySegment polarity protein dishevelled homolog DVL 2 antibodySegment polarity protein dishevelled homolog DVL-2 antibodySegment polarity protein dishevelled homolog DVL2 antibody

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Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] images

• 

  Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)
  All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution
  
  Lane 1 : F9 cell lysate untreated
  Lane 2 : F9 cell lysate treated with Alkaline Phosphatase
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
  
  Predicted band size : 78 kDa
  Observed band size : 95 kDa (why is the actual band size different from the predicted?)