Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)

Overview

• Product nameAnti-Dishevelled 3 antibody [EP1991Y]See all Dishevelled 3 primary antibodies ...
• Description
  Rabbit monoclonal [EP1991Y] to Dishevelled 3
• Tested applicationsWB, ICC, Flow Cyt, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide corresponding to residues near the C-terminal of human Dishevelled 3.

• Positive controlK562 cell lysate and human brain tissue
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEP1991Y
• IsotypeIgG
• Research Areas
  • Stem Cells
  • Signaling Pathways
  • Notch
  • Cytoplasmic

  • Stem Cells
  • Signaling Pathways
  • Wnt
  • Cytoplasmic

  • Cardiovascular
  • Heart
  • Cardiogenesis
  • Cardiomyocyte precursors

  • Neuroscience
  • Neurology process
  • Neurogenesis

Applications

Target

• FunctionMay play a role in the signal transduction pathway mediated by multiple Wnt genes.
• Sequence similaritiesBelongs to the DSH family.
  Contains 1 DEP domain.
  Contains 1 DIX domain.
  Contains 1 PDZ (DHR) domain.
• Post-translational
  modificationsUbiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.
• Cellular localizationCytoplasm.

Target information above from: UniProt accession Q92997 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1857 Human
  • Entrez Gene: 13544 Mouse
  • Entrez Gene: 303811 Rat
  • Omim: 601368 Human
  • SwissProt: Q92997 Human
  • SwissProt: Q61062 Mouse
  • Unigene: 388116 Human
  • Unigene: 247259 Mouse

  • Unigene: 272443 Mouse
  • Unigene: 219497 Rat

  see all

• Alternative names
  Dishevelled 3 (homologous to Drosophila dsh) antibodyDishevelled 3 antibodyDishevelled 3, dsh homolog (Drosophila) antibody

  Dishevelled, dsh homolog 3 (Drosophila) antibodyDishevelled-3 antibodyDSH homolog 3 antibodydvl3 antibodyDVL3_HUMAN antibodyKIAA0208 antibodyOTTHUMP00000210777 antibodyOTTHUMP00000210778 antibodySegment polarity protein dishevelled homolog DVL 3 antibodySegment polarity protein dishevelled homolog DVL-3 antibody

  see all

Anti-Dishevelled 3 antibody [EP1991Y] images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dishevelled 3 antibody [EP1991Y] (ab76081)
  Immunohistochemical staining of paraffin-embedded human brain tissue, using ab76081, at 1/100-1/250 dilution.
• 

  Western blot - Dishevelled 3 antibody [EP1991Y] (ab76081)
  Anti-Dishevelled 3 antibody [EP1991Y] (ab76081) at 1/1000 dilution + K562 cell lysate at 10 µg
  
  Secondary
  goat anti-rabbit HRP labelled IgG at 1/2000 dilution
  
  Predicted band size : 78 kDa
  Observed band size : 95 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - Dishevelled 3 antibody [EP1991Y] (ab76081)
  ICC/IF image of ab76081 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76081, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Flow Cytometry-Anti-Dishevelled 3 antibody [EP1991Y](ab76081)
  Overlay histogram showing HeLa cells stained with ab76081 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76081, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.