RabMAb

Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)

Overview

  • Product nameAnti-Dishevelled 3 antibody [EP1991Y]See all Dishevelled 3 primary antibodies ...
  • Description
    Rabbit monoclonal [EP1991Y] to Dishevelled 3
  • Tested applicationsWB, ICC, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminal of human Dishevelled 3.

  • Positive control
    • K562 cell lysate and human brain tissue
  • General notes

    Produced under U.S. Patent No. 5,675,063.

    An immunizing peptide is available for this antibody as ab191381.

     

Properties

Applications

Our Abpromise guarantee covers the use of ab76081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 78 kDa.Can be blocked with Dishevelled 3 peptide (ab191381).
ICC 1/100 - 1/250.
Flow Cyt 1/30.
IHC-P Use at an assay dependent dilution.
ICC/IF Use a concentration of 5 µg/ml.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionMay play a role in the signal transduction pathway mediated by multiple Wnt genes.
    • Sequence similaritiesBelongs to the DSH family.
      Contains 1 DEP domain.
      Contains 1 DIX domain.
      Contains 1 PDZ (DHR) domain.
    • Post-translational
      modifications
      Ubiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.
    • Cellular localizationCytoplasm.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dishevelled 3 (homologous to Drosophila dsh) antibody
      • Dishevelled 3 antibody
      • Dishevelled 3, dsh homolog (Drosophila) antibody
      • Dishevelled, dsh homolog 3 (Drosophila) antibody
      • Dishevelled-3 antibody
      • DSH homolog 3 antibody
      • dvl3 antibody
      • DVL3_HUMAN antibody
      • KIAA0208 antibody
      • OTTHUMP00000210777 antibody
      • OTTHUMP00000210778 antibody
      • Segment polarity protein dishevelled homolog DVL 3 antibody
      • Segment polarity protein dishevelled homolog DVL-3 antibody
      see all

    Anti-Dishevelled 3 antibody [EP1991Y] images

    • Immunohistochemical staining of paraffin-embedded human brain tissue, using ab76081, at 1/100-1/250 dilution.
    • Anti-Dishevelled 3 antibody [EP1991Y] (ab76081) at 1/1000 dilution + K562 cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP labelled IgG at 1/2000 dilution

      Predicted band size : 78 kDa
      Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab76081 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76081, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Overlay histogram showing HeLa cells stained with ab76081 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76081, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References for Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)

    This product has been referenced in:
    • Burcklé C  et al. Control of the Wnt pathways by nephrocystin-4 is required for morphogenesis of the zebrafish pronephros. Hum Mol Genet 20:2611-27 (2011). IHC (PFA fixed) . Read more (PubMed: 21498478) »

    See 1 Publication for this product

    Product Wall

    Abreviews
    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (12 % SDS PAGE gel)
    Sample Human Cell lysate - whole cell (HEK 293T cells)
    Specification HEK 293T cells
    Treatment transfected with Dvl-3 plasmid
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted May 23 2013

    Merci d'avoir pris le temps de remplir notre questionnaire. Je suis désolée que vous avez eu des difficultés à obtenir de bons résultats avec ce nouveau lot.

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    Je suis désolée d'apprendre que un nouveau lot d'ab76081 ne vous donne pas de bons résultats.
    Veuillez trouver ci-joint le questionnaire discuté au téléphone qui nous permet de regrouper le plus d'informations le protocole que vous avez utilisé av...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"