RabMAb

Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)

Overview

  • Product nameAnti-Dishevelled 3 antibody [EP1991Y]See all Dishevelled 3 primary antibodies ...
  • Description
    Rabbit monoclonal [EP1991Y] to Dishevelled 3
  • Tested applicationsWB, ICC, Flow Cyt, IHC-P, ICC/IF more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide corresponding to residues near the C-terminal of human Dishevelled 3.

  • Positive control
    • K562 cell lysate and human brain tissue
  • General notesProduced under U.S. Patent No. 5,675,063.

Properties

Applications

Our Abpromise guarantee covers the use of ab76081 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/500 - 1/1000. Predicted molecular weight: 78 kDa.
ICC 1/100 - 1/250.
Flow Cyt 1/30.
IHC-P Use at an assay dependent dilution.
ICC/IF Use a concentration of 5 µg/ml.
  • Application notesIs unsuitable for IP.
  • Target

    • FunctionMay play a role in the signal transduction pathway mediated by multiple Wnt genes.
    • Sequence similaritiesBelongs to the DSH family.
      Contains 1 DEP domain.
      Contains 1 DIX domain.
      Contains 1 PDZ (DHR) domain.
    • Post-translational
      modifications
      Ubiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.
    • Cellular localizationCytoplasm.
    • Target information above from: UniProt accession Q92997 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links
    • Alternative names
      • Dishevelled 3 (homologous to Drosophila dsh) antibody
      • Dishevelled 3 antibody
      • Dishevelled 3, dsh homolog (Drosophila) antibody
      • Dishevelled, dsh homolog 3 (Drosophila) antibody
      • Dishevelled-3 antibody
      • DSH homolog 3 antibody
      • dvl3 antibody
      • DVL3_HUMAN antibody
      • KIAA0208 antibody
      • OTTHUMP00000210777 antibody
      • OTTHUMP00000210778 antibody
      • Segment polarity protein dishevelled homolog DVL 3 antibody
      • Segment polarity protein dishevelled homolog DVL-3 antibody
      see all

    Anti-Dishevelled 3 antibody [EP1991Y] images

    • Immunohistochemical staining of paraffin-embedded human brain tissue, using ab76081, at 1/100-1/250 dilution.
    • Anti-Dishevelled 3 antibody [EP1991Y] (ab76081) at 1/1000 dilution + K562 cell lysate at 10 µg

      Secondary
      goat anti-rabbit HRP labelled IgG at 1/2000 dilution

      Predicted band size : 78 kDa
      Observed band size : 95 kDa (why is the actual band size different from the predicted?)
    • ICC/IF image of ab76081 stained PC12 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76081, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    • Overlay histogram showing HeLa cells stained with ab76081 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab76081, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

    References for Anti-Dishevelled 3 antibody [EP1991Y] (ab76081)

    This product has been referenced in:
    • Burcklé C  et al. Control of the Wnt pathways by nephrocystin-4 is required for morphogenesis of the zebrafish pronephros. Hum Mol Genet 20:2611-27 (2011). IHC (PFA fixed) . Read more (PubMed: 21498478) »

    See 1 Publication for this product

    Product Wall

    Abreviews
    Application Western blot
    Loading amount 20 µg
    Gel Running Conditions Reduced Denaturing (12 % SDS PAGE gel)
    Sample Human Cell lysate - whole cell (HEK 293T cells)
    Specification HEK 293T cells
    Treatment transfected with Dvl-3 plasmid
    Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Username

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    Verified customer

    Submitted May 23 2013

    Merci d'avoir pris le temps de remplir notre questionnaire. Je suis désolée que vous avez eu des difficultés à obtenir de bons résultats avec ce nouveau lot.

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    Je suis désolée d'apprendre que un nouveau lot d'ab76081 ne vous donne pas de bons résultats.
    Veuillez trouver ci-joint le questionnaire discuté au téléphone qui nous permet de regrouper le plus d'informations le protocole que vous avez utilisé av...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"