Overview
- Product nameAnti-Dishevelled 3 antibodySee all Dishevelled 3 primary antibodies ...
- DescriptionRabbit polyclonal to Dishevelled 3
- Specificityab22617 recognises dishevelled 1. It doesn't recognise dishevelled 2 or dishevelled 3. By BLAST search of the immunogen aminoacids sequence, ab22617 may recognise human Hypothetical protein DKFZp586M1622 and human Protein kinase C-binding protein RACK8.
- Tested applicationsWB more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
Synthetic peptide: C+GSDRRKEKDPKAGDS conjugated to KLH by a MBS linker, corresponding to amino acids 582/596 of Human Dishevelled 3.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 10mM Sodium Azide
Constituents: PBS, pH 7.4 -
Concentration information loading... - Purification notesab22617 is rabbit antiserum partially purified by caprylic acid and ammonium sulphate precipitation.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab22617 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB |
Note: there is a weak reactivity observed with dishevelled 1 and dishevelled 2 that is due to protein protein interaction, and is not abolished following peptide preadsorption.
Note: dilute ab22617 to working strength in 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat anti rabbit IgG linker antibody is to be used).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionMay play a role in the signal transduction pathway mediated by multiple Wnt genes.
- Sequence similaritiesBelongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain. - Post-translational
modificationsUbiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. - Cellular localizationCytoplasm.
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Database links
- Entrez Gene: 1857 Human
- Entrez Gene: 13544 Mouse
- Omim: 601368 Human
- SwissProt: Q92997 Human
- SwissProt: Q61062 Mouse
- Unigene: 388116 Human
- Unigene: 247259 Mouse
- Unigene: 272443 Mouse
Target information above from: UniProt accession
Q92997
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Dishevelled 3 (homologous to Drosophila dsh) antibodyDishevelled 3 antibodyDishevelled 3, dsh homolog (Drosophila) antibody
- Dishevelled, dsh homolog 3 (Drosophila) antibodyDishevelled-3 antibodyDSH homolog 3 antibodyDVL3 antibodyDVL3_HUMAN antibodyKIAA0208 antibodyKIAA0208 antibodyOTTHUMP00000210777 antibodyOTTHUMP00000210778 antibodySegment polarity protein dishevelled homolog DVL 3 antibodySegment polarity protein dishevelled homolog DVL 3 antibodySegment polarity protein dishevelled homolog DVL-3 antibody
see all
References for Anti-Dishevelled 3 antibody (ab22617)
ab22617 has not yet been referenced specifically in any publications.
