Overview
- Product nameAnti-Dishevelled 3 antibodySee all Dishevelled 3 primary antibodies ...
- DescriptionRabbit polyclonal to Dishevelled 3
- Tested applicationsWB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human Dishevelled 3.
(Peptide available as ab108471.)
- Positive controlThis antibody gave a positive signal in the following lysates: Ovary (Human) Tissue Lysate; Heart (Human) Tissue Lysate
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab93850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 mg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).Can be blocked with Dishevelled 3 peptide (ab108471). |
Target
- FunctionMay play a role in the signal transduction pathway mediated by multiple Wnt genes.
- Sequence similaritiesBelongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain. - Post-translational
modificationsUbiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. - Cellular localizationCytoplasm.
-
Database links
- Entrez Gene: 1857 Human
- Entrez Gene: 13544 Mouse
- Entrez Gene: 303811 Rat
- Omim: 601368 Human
- SwissProt: Q92997 Human
- SwissProt: Q61062 Mouse
- Unigene: 388116 Human
- Unigene: 247259 Mouse
- Unigene: 272443 Mouse
- Unigene: 219497 Rat
see all
Target information above from: UniProt accession
Q92997
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- Dishevelled 3 (homologous to Drosophila dsh) antibodyDishevelled 3 antibodyDishevelled 3, dsh homolog (Drosophila) antibody
- Dishevelled, dsh homolog 3 (Drosophila) antibodyDishevelled-3 antibodyDSH homolog 3 antibodyDVL3 antibodyDVL3_HUMAN antibodyKIAA0208 antibodyOTTHUMP00000210777 antibodyOTTHUMP00000210778 antibodySegment polarity protein dishevelled homolog DVL 3 antibodySegment polarity protein dishevelled homolog DVL-3 antibody
see all
Anti-Dishevelled 3 antibody images
-
Western blot - Dishevelled 3 antibody (ab93850)All lanes : Anti-Dishevelled 3 antibody (ab93850) at 1 µg/ml
Lane 1 :Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)
Lane 2 :Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 78 kDa
Observed band size : 78 kDa
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 3 minutes
References for Anti-Dishevelled 3 antibody (ab93850)
ab93850 has not yet been referenced specifically in any publications.
