Anti-Dlx5 antibody (ab64827)
Key features and details
- Rabbit polyclonal to Dlx5
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Dlx5 antibody
See all Dlx5 primary antibodies -
Description
Rabbit polyclonal to Dlx5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide derived from an internal sequence of human Dlx5.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab64827 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64827 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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WB |
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa).
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Notes |
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IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. Detects a band of approximately 32 kDa (predicted molecular weight: 32 kDa). |
Target
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Cellular localization
Nuclear -
Database links
- Entrez Gene: 1749 Human
- Omim: 600028 Human
- SwissProt: P56178 Human
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Alternative names
- Distal less homeo box 5 antibody
- Distal less homeobox 5 antibody
- Dlx 5 antibody
see all
Images
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All lanes : Anti-Dlx5 antibody (ab64827) at 1/500 dilution
Lane 1 : HeLa cell extract at 30 µg
Lane 2 : 293 cell extract at 30 µg
Lane 3 : COLO205 cell extract at 30 µg
Lane 4 : COLO205 cell extract at 30 µg
plus immunising peptide at 10 µg
Predicted band size: 32 kDa
Observed band size: 32 kDa -
ab64827, at 1/50 dilution, staining Dlx5 in paraffin embedded human lung carcinoma tissue by Immunohistochemistry in the absence or presence of the immunising peptide.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (4)
ab64827 has been referenced in 4 publications.
- Yanar K et al. Contribution of Human Trophoblast Progenitor Cells to Neurogenesis in Rat Focal Cerebral Ischemia Model. Brain Inj 35:850-862 (2021). PubMed: 33780298
- Son C et al. Different Responsiveness of Alveolar Bone and Long Bone to Epithelial-Mesenchymal Interaction-Related Factor. JBMR Plus 4:e10382 (2020). PubMed: 32803111
- Nestor MW et al. Characterization of a subpopulation of developing cortical interneurons from human iPSCs within serum-free embryoid bodies. Am J Physiol Cell Physiol 308:C209-19 (2015). ICC/IF ; Mouse . PubMed: 25394470
- Ohlemacher SK et al. Generation of highly enriched populations of optic vesicle-like retinal cells from human pluripotent stem cells. Curr Protoc Stem Cell Biol 32:1H.8.1-1H.8.20 (2015). PubMed: 25640818