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Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)

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Overview

Product name

Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade
See all Dnmt1 products (15) ...

Description

Mouse monoclonal [60B1220.1] to Dnmt1 - ChIP Grade

Tested applications

WB, IHC-P, ChIP, IPmore details

Cross reactivity

Reacts with

Mouse, Human, Zebrafish

Predicted to work with

Sheep, Goat, Cow, Pig

Immunogen

Synthetic peptide: EKDDREDKENAFKR, corresponding to amino acids 637-650 of Human Dnmt1 (NP_001370).

EKDDREDKENAFKR

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

Human kidney tissue (IHC-P) Mouse embryonic stem and NIH 3T3 cell lysates (WB)

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.05% Sodium Azide
Constituents: 0.05% BSA, PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

60B1220.1

Isotype

IgG1

Light chain type

kappa

Research areas

Epigenetics and Nuclear Signaling >> ChIP'ing antibodies >> ChIP'ing antibodies
Epigenetics and Nuclear Signaling >> DNA methylation >> DNMTs

  • Western blot - Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)Western blot - Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453) image (enlarge)

  • Western blot - Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)Western blot - Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab92453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ChIP: Use at an assay dependent dilution. See PubMed: 15548683 reference below.
IHC-P: Use at a concentration of 1 - 2 µg/ml.
IP: Use at an assay dependent dilution. See PubMed: 18539602 reference below.
WB: Use at a concentration of 2 - 4 µg/ml. Predicted molecular weight: 183 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.

Tissue specificity

Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.

Sequence similarities

Belongs to the C5-methyltransferase family.
Contains 2 BAH domains.
Contains 1 CXXC-type zinc finger.

Domain

The N-terminal part is required for homodimerization and acts as a regulatory domain.

Post-translational
modifications

Sumoylated; sumoylation increases activity.

Cellular localization

Nucleus.

Target information above from: UniProt accessionP26358 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • AIM antibody
  • CXXC-type zinc finger protein 9 antibody
  • CXXC9 antibody
  • DNA (cytosine 5 ) methyltransferase 1 antibody
  • DNA (cytosine-5)-methyltransferase 1 antibody
  • DNA methyltransferase 1 antibody
  • DNA methyltransferase HsaI antibody
  • DNA methyltransferase M.HsaI. antibody
  • DNA MTase antibody
  • DNA MTase HsaI antibody
  • DNMT 1 antibody
  • DNMT antibody
  • Dnmt1 antibody
  • Dnmt1 antibody
  • DNMT1_HUMAN antibody
  • FLJ16293 antibody
  • M.HsaI antibody
  • MCMT antibody
  • MGC104992 antibody
see all

Database links

see all

Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade images:

  Western blot - Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)

Western blot - Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)

All lanes : Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)

Lane 1 : 2102EP (human embryonic carcinoma) with absence of blocking peptide
Lane 2 : 2102EP (human embryonic carcinoma) with blocking peptide
Lane 3 : Recombinant human Dnmt1 protein with absence of blocking peptide
Lane 4 : Recombinant human Dnmt1 protein with blocking peptide
Lane 5 : NIH 3T3 (mouse embryonic fibroblast) with absence of blocking peptide
Lane 6 : NIH 3T3 (mouse embryonic fibroblast) with blocking peptide
Lane 7 : D3 (mouse embryonic stem cell) with absence of blocking peptide
Lane 8 : D3 (mouse embryonic stem cell) with blocking peptide

Secondary
Goat anti-Mouse IgG HRP-conjugated secondary antibody

Predicted band size : 183 kDa
Observed band size : 183 kDa
Additional bands at : 220 kDa,35 kDa,75 kDa. We are unsure as to the identity of these extra bands.

  Western blot - Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)

Western blot - Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)

Lane 1 : Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453) at 2 µg/ml
Lane 2 : Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453) at 0.2 µg/ml

Lane 1 : Mouse ES cell lysate
Lane 2 : Mouse NIH3T3 cell lysate

Secondary
Goat anti-Mouse Ig HRP conjugated

Predicted band size : 183 kDa

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)

ab92453, at 2 µg/ml, staining Dnmt1 on formalin fixed, paraffin embedded medullar kidney tissue sections by Immunohistochemistry.

References for Anti-Dnmt1 antibody [60B1220.1] - ChIP Grade (ab92453)

This product has been referenced in:

  • Guastafierro T  et al. CCCTC-binding factor activates PARP-1 affecting DNA methylation machinery. J Biol Chem 283:21873-80 (2008). WB, IP; Mouse.Read more (PubMed: 18539602) »
  • Rai K  et al. Zebra fish Dnmt1 and Suv39h1 regulate organ-specific terminal differentiation during development. Mol Cell Biol 26:7077-85 (2006). Zebrafish.Read more (PubMed: 16980612) »

See all 8 publications for this product

Publishing research using ab92453? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"