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Product name
Anti-Dnmt1 antibody - ChIP Grade
See all Dnmt1 products (15) ...
Description
Rabbit polyclonal to Dnmt1 - ChIP Grade
Tested applications
WB, IP, ICC/IF, ChIPmore details
Cross reactivity
Reacts with
Mouse, Human, Xenopus laevis
Immunogen
Recombinant fragment, corresponding to amino acids 1037-1386 of Mouse Dnmt1, purified by SDS-PAGE.
Positive control
Murine erythrolekemia cells MEL11A2
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium azide
Constituents: 50% Glycerol, PBS
Note: Traces of Ammonium sulphate
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab87656 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.2 - 1 µg/ml. Predicted molecular weight: 183 kDa.
IP: Use at an assay dependent dilution. Especially good for denatured protein.
ICC/IF: 1/2000 - 1/5000. Background can be lowered by treatment of sample with SDS/TritonX-100 mixture (1:5 weight ratio).
ChIP: Use at an assay dependent dilution.
Target
Function
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Tissue specificity
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 2 BAH domains.
Contains 1 CXXC-type zinc finger.
Domain
The N-terminal part is required for homodimerization and acts as a regulatory domain.
Post-translational
modifications
Sumoylated; sumoylation increases activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP26358
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AIM antibody
- CXXC-type zinc finger protein 9 antibody
- CXXC9 antibody
see all
Database links
- Entrez Gene: 1786 Human
- Entrez Gene: 13433 Mouse
- Omim: 126375 Human
- SwissProt: P26358 Human
- SwissProt: P13864 Mouse
- Unigene: 202672 Human
- Unigene: 128580 Mouse
Anti-Dnmt1 antibody - ChIP Grade images:
Western blot - Dnmt1 antibody (ab87656)
All lanes : Anti-Dnmt1 antibody - ChIP Grade (ab87656) at 1 µg/ml
Lane 1 : lysate from Mouse erythrolekemia cells MEL11A2
Lane 2 : lysate from untransfected Mouse myoblast cells C2C12
Lane 3 : lysate from C2C12 cells transfected with Dnmt1 overexpressing plasmid
Lane 4 : lysate from C2C12 cells transfected with negative control plasmid
Predicted band size : 183 kDa
Immunoprecipitation - Dnmt1 antibody (ab87656)
Lysate from Mouse erythrolekemia cells MEL11A2 (lane 1), untransfected Mouse myoblast cells C2C12 (lane 2) , C2C12 cells transfected with Dnmt1 overexpressing plasmid (lane 3) and C2C12 cells transfected with negative control plasmid (lane 4) were immunoprecipitated under denaturing conditions using ab87656.
The immunoprecipitae was subsequently loaded on a gel and probed with ab87656 at 1yg/ml.
References for Anti-Dnmt1 antibody - ChIP Grade (ab87656)
ab87656 has not yet been referenced specifically in any publications.
Publishing research using ab87656? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"