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Product name
Anti-Dnmt1 antibody
See all Dnmt1 products (15) ...
Description
Rabbit polyclonal to Dnmt1
Specificity
Detects DNA methyltransferase 1 (Dmnt1). We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide: AKGPAKRKPQEESE, corresponding to amino acids 171-185 of Human Dnmt 1.
AKGPAKRKPQEESE
Positive control
HeLa cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA, 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA methylation >> DNMTs
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Western blot - Dnmt1 antibody (ab2849)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab2849 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notes
WB: Use at a concentration of 0.5 - 1 µg/ml. Detects a band of approximately 180 kDa (predicted molecular weight: 183 kDa). A doublet between 40 and 45 kDa is also seen on Western blots using this antibody. Can be blocked with Dnmt 1 peptide
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Tissue specificity
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 2 BAH domains.
Contains 1 CXXC-type zinc finger.
Domain
The N-terminal part is required for homodimerization and acts as a regulatory domain.
Post-translational
modifications
Sumoylated; sumoylation increases activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP26358
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AIM antibody
- CXXC-type zinc finger protein 9 antibody
- CXXC9 antibody
see all
Database links
- Entrez Gene: 1786 Human
- Omim: 126375 Human
- SwissProt: P26358 Human
- Unigene: 202672 Human
Anti-Dnmt1 antibody images:
Western blot - Dnmt1 antibody (ab2849)
Predicted band size : 183 kDa
Rabbit polyclonal to Dnmt1 on HeLa cell nuclear extract.
References for Anti-Dnmt1 antibody (ab2849)
ab2849 has not yet been referenced specifically in any publications.
Publishing research using ab2849? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"