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Product name
Anti-Dnmt1 antibody
See all Dnmt1 products (15) ...
Description
Rabbit polyclonal to Dnmt1
Specificity
Dnmt 1. Will recognize the splice isoforms B and C.
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow
Immunogen
Synthetic peptide conjugated to KLH, corresponding to within the last 100 amino acids of Human Dnmt 1.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
Purified through a protein G column and eluted out with both high and low pH buffers, neutralized immediately after elution, followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA methylation >> DNMTs
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Western blot - Anti-Dnmt1 antibody (ab4870)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab4870 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA
ELISA: 1/1000.ELISA: 1/1000.
WB: 1/100 - 1/500. Detects a band of approximately 183 kDa.
Target
Function
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Tissue specificity
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 2 BAH domains.
Contains 1 CXXC-type zinc finger.
Domain
The N-terminal part is required for homodimerization and acts as a regulatory domain.
Post-translational
modifications
Sumoylated; sumoylation increases activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP26358
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AIM antibody
- CXXC-type zinc finger protein 9 antibody
- CXXC9 antibody
see all
Database links
- Entrez Gene: 281119 Cow
- Entrez Gene: 1786 Human
- Entrez Gene: 13433 Mouse
- Entrez Gene: 84350 Rat
- Omim: 126375 Human
- SwissProt: Q24K09 Cow
- SwissProt: P26358 Human
- SwissProt: P13864 Mouse
see all
Anti-Dnmt1 antibody images:
Western blot - Anti-Dnmt1 antibody (ab4870)
ab4870 used in Western blot to detect Dnmt1 in Jurkat cell lysate.
References for Anti-Dnmt1 antibody (ab4870)
This product has been referenced in:
- Sarkar S et al. Histone Deacetylase Inhibitors Reverse CpG Methylation by Regulating DNMT1 through ERK Signaling. Anticancer Res 31:2723-32 (2011). WB; Human.Read more (PubMed: 21868513) »
See 1 publication for this product
Publishing research using ab4870? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"