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Product name
Anti-Dnmt1 antibody
See all Dnmt1 products (15) ...
Description
Rabbit polyclonal to Dnmt1
Specificity
ab87656 is recommended for DNMT1 detection in WB.
Tested applications
IP, ICC/IF, WBmore details
Cross reactivity
Reacts with
Mouse, Human, Xenopus laevis
Immunogen
Highly purified recombinant Mouse Dnmt1 (amino acids 1-248), soluble form
Positive control
WB: HeLa cell nuclear extracts. ICC/IF: HeLa cells.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, traces of Ammonium sulphate, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Useful for analysis of the protein complex containing Dnmt1.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA methylation >> DNMTs
Applications
Show applications key
Our Abpromise guarantee covers the use of ab87654 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IP: Use at an assay dependent dilution. 1 ug of the antibody can precipitates 0.5~1.0 ug of Dnmt1. Antibody works best for native Dnmt1.
ICC/IF: 1/5000.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 183 kDa (predicted molecular weight: 183 kDa).
Target
Function
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Tissue specificity
Ubiquitous; highly expressed in fetal tissues, heart, kidney, placenta, peripheral blood mononuclear cells, and expressed at lower levels in spleen, lung, brain, small intestine, colon, liver, and skeletal muscle. Isoform 2 is less expressed than isoform 1.
Sequence similarities
Belongs to the C5-methyltransferase family.
Contains 2 BAH domains.
Contains 1 CXXC-type zinc finger.
Domain
The N-terminal part is required for homodimerization and acts as a regulatory domain.
Post-translational
modifications
Sumoylated; sumoylation increases activity.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP26358
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AIM antibody
- CXXC-type zinc finger protein 9 antibody
- CXXC9 antibody
see all
Database links
- Entrez Gene: 1786 Human
- Entrez Gene: 13433 Mouse
- Omim: 126375 Human
- SwissProt: P26358 Human
- SwissProt: P13864 Mouse
- Unigene: 202672 Human
- Unigene: 128580 Mouse
Anti-Dnmt1 antibody images:
Immunocytochemistry/ Immunofluorescence - Dnmt1 antibody (ab87654)
ab87654 being used to indicate the subcellular localization of Dnmt1 in mouse embryonic stem cells (E14) during cell cycle progression.
Western blot - Dnmt1 antibody (ab87654)
All lanes : Anti-Dnmt1 antibody (ab87654) at 1 µg/ml
Lane 1 : Tagged-NP 95 + BirA Input
Lane 2 : Tagged-NP 95 + BirA Beads
Lane 3 : BirA Input
Lane 4 : BirA Beads
Predicted band size : 183 kDa
Observed band size : 183 kDa
Association of Np95 with Dnmt1 and Pcna in HeLa cell nuclear extracts. Human NP95 was tagged by biotin-binding domain and stably expressed in HeLa cells together with the E. coli BirA biotin ligase gene. Biotylated Np95 was captured by streptoavidin beads. The captured proteins were eluted from the beads and analyzed by western bloting using antibodies against Dnmt1 (ab87654) and Pcna.
Western blot - Dnmt1 antibody (ab87654)
All lanes : Anti-Dnmt1 antibody (ab87654) at 1 µg/ml
Lane 1 : Dnmt1 1+/+ (J1) (nuclear extract)
Lane 2 : Dnmt1 1-/- (36-2D6) (nuclear extract)
Lane 3 : Tagged-NP95 + BirA (0.1M KCl fraction)
Lane 4 : BirA (0.1M KCl fraction)
Predicted band size : 183 kDa
Catalytic activity of NP95 associated Dnmt1 in HeLa cells. The presence of Dnmt1 in the NP95 complexes was confirmed by western blot.
References for Anti-Dnmt1 antibody (ab87654)
ab87654 has not yet been referenced specifically in any publications.
Publishing research using ab87654? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"