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Anti-Dnmt3a antibody [64B814] (ab13887)

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Overview

Product name

Anti-Dnmt3a antibody [64B814]
See all Dnmt3a products (10) ...

Description

Mouse monoclonal [64B814] to Dnmt3a

Tested applications

WB, IF, ICCmore details

Cross reactivity

Reacts with

Mouse, Human

Immunogen

Bacteria expressed HIS-tag recombinant mouse Dnmt3a.

Positive control

293 cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Monoclonal

Clone number

64B814

Isotype

IgG1

Research areas

Epigenetics and Nuclear Signaling >> DNA methylation >> DNMTs

  • Western blot - Dnmt3a antibody [ 64B814] (ab13887)Western blot - Dnmt3a antibody [ 64B814] (ab13887) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab13887 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ShowHide1 Image

    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 2 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).

  • ShowHide

    IF

     IF: Use at an assay depe...Read more →

    IF: Use at an assay dependent dilution.

  • ShowHide

    ICC

     ICC: Use at an assay dep...Read more →

    ICC: Use at an assay dependent dilution.

Target

Function

Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.

Tissue specificity

Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Sequence similarities

Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain.

Domain

The PWWP domain is essential for targeting to pericentric heterochromatin.

Post-translational
modifications

Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.

Cellular localization

Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.

Target information above from: UniProt accessionQ9Y6K1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DNA (cytosine 5 ) methyltransferase 3 alpha antibody
  • DNA (cytosine 5) methyltransferase 3 alpha antibody
  • DNA (cytosine 5) methyltransferase 3A antibody
  • DNA (cytosine-5)-methyltransferase 3A antibody
  • DNA cytosine methyltransferase 3A2 antibody
  • DNA methyltransferase 3a antibody
  • DNA methyltransferase 3a antibody
  • DNA methyltransferase HsaIIIA antibody
  • DNA MTase HsaI antibody
  • DNA MTase HsaI antibody
  • DNA MTase HsaIIIA antibody
  • DNM3A_HUMAN antibody
  • DNMT 3a antibody
  • DNMT 3a antibody
  • DNMT antibody
  • DNMT antibody
  • Dnmt3a antibody
  • Dnmt3a antibody
  • DNMT3A2 antibody
  • M HsaIIIA antibody
  • M.HsaIIIA antibody
  • MCMT antibody
  • MCMT antibody
  • Methyl CpG binding domain protein 3a antibody
  • Methyl CpG binding domain protein 3a antibody
  • OTTHUMP00000201149 antibody
see all

Database links

Anti-Dnmt3a antibody [64B814] images:

  Western blot - Dnmt3a antibody [ 64B814] (ab13887)

Western blot - Dnmt3a antibody [ 64B814] (ab13887)



Predicted band size : 102 kDa


Western blot analysis for Dnmt3a using ab13887 at 2 ug/ml against 10 ug of 293 cell lysate transfected with either mouse Dnmt3a (lane 1) or mouse Dnmt3b (lane 2).

References for Anti-Dnmt3a antibody [64B814] (ab13887)

This product has been referenced in:

  • Sato N  et al. The orphan nuclear receptor GCNF recruits DNA methyltransferase for Oct-3/4 silencing. Biochem Biophys Res Commun 344:845-51 (2006). WB; Mouse.Read more (PubMed: 16631596) »

See 1 publication for this product

Publishing research using ab13887? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"