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Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Dnmt3a.
(Peptide available as ab26339.)
Our Abpromise guarantee covers the use of ab23565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-P||1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Can be blocked with Human Dnmt3a peptide (ab26339).|
|IHC||Use at an assay dependent concentration. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.|
ab23565 staining Dnmt3a in mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/300) for 24 hours at 4°C. A HRP-conjugated anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"