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Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Mouse Dnmt3a.
(Peptide available as ab26339.)
Our Abpromise guarantee covers the use of ab23565 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 10 µg/ml.|
|IHC-P||1/300. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 102 kDa.|
ab23565 staining Dnmt3a in mouse lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 25°C; antigen retrieval was by heat mediation. Samples were incubated with primary antibody (1/300) for 24 hours at 4°C. A HRP-conjugated anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody.
ab23565 recognizes the tagged Dnmt3a recombinant protein which has an expected molecular weight of 120 kDa.
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