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Anti-Dnmt3a antibody (ab4897)

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Overview

Product name

Anti-Dnmt3a antibody
See all Dnmt3a products (10) ...

Description

Rabbit polyclonal to Dnmt3a

Tested applications

ICC/IF, IHC-P, ELISA, WB, IHC-Frmore details

Cross reactivity

Reacts with

Human

Immunogen

Synthetic peptide: DERTRERLVYEVRQK conjugated to KLH, corresponding to amino acids 469 - 483 of Human Dnmt3a.

DERTRERLVYEVRQK

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.09% Sodium Azide
Constituents: PBS

Concentration

Concentration information loading...

Purity

Protein G purified

Purification notes

Purified through a protein G column and eluted out with both high and low pH buffers, neutralized immediately after elution, followed by dialysis against PBS.

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> DNA methylation >> DNMTs

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dnmt3a antibody (ab4897)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dnmt3a antibody (ab4897) image (enlarge)

  • Western blot - Dnmt3a antibody (ab4897)Western blot - Dnmt3a antibody (ab4897) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab4897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Target

Function

Required for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.

Tissue specificity

Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.

Sequence similarities

Belongs to the C5-methyltransferase family.
Contains 1 ADD domain.
Contains 1 GATA-type zinc finger.
Contains 1 PHD-type zinc finger.
Contains 1 PWWP domain.

Domain

The PWWP domain is essential for targeting to pericentric heterochromatin.

Post-translational
modifications

Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.

Cellular localization

Nucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.

Target information above from: UniProt accessionQ9Y6K1 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • DNA (cytosine 5 ) methyltransferase 3 alpha antibody
  • DNA (cytosine 5) methyltransferase 3 alpha antibody
  • DNA (cytosine 5) methyltransferase 3A antibody
  • DNA (cytosine-5)-methyltransferase 3A antibody
  • DNA cytosine methyltransferase 3A2 antibody
  • DNA methyltransferase 3a antibody
  • DNA methyltransferase HsaIIIA antibody
  • DNA MTase HsaI antibody
  • DNA MTase HsaIIIA antibody
  • DNM3A_HUMAN antibody
  • DNMT 3a antibody
  • DNMT antibody
  • DNMT antibody
  • Dnmt3a antibody
  • DNMT3A2 antibody
  • M HsaIIIA antibody
  • M.HsaIIIA antibody
  • MCMT antibody
  • Methyl CpG binding domain protein 3a antibody
  • OTTHUMP00000201149 antibody
see all

Database links

Anti-Dnmt3a antibody images:

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dnmt3a antibody (ab4897)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Dnmt3a antibody (ab4897)

ab4897 staining Dnmt3a in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

  Western blot - Dnmt3a antibody (ab4897)

Western blot - Dnmt3a antibody (ab4897)

Anti-Dnmt3a antibody (ab4897) + T247-D cell lysate (Top)

Secondary
HRP-conjugated anti-rabbit

Observed band size : 101 kDa (why is the actual band size different from the predicted?)


(Bottom) Lysates from thymus tissue after radiation were subjected to WB using antibody against DNMT3a. CT, control animals; FR, animals subjected to fractionated exposure; AC, acutely exposed animals. All sample loading was normalized to protein content. Representative Western blots from three independent experiments are shown; each lane represents a protein extract of a thymus of one animal.

References for Anti-Dnmt3a antibody (ab4897)

This product has been referenced in:

  • Chik F & Szyf M Effects of specific DNMT gene depletion on cancer cell transformation and breast cancer cell invasion; toward selective DNMT inhibitors. Carcinogenesis 32:224-32 (2011). WB; Human.Read more (PubMed: 20980350) »

See 1 publication for this product

Publishing research using ab4897? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"