Overview

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified through a protein G column and eluted out with both high and low pH buffers, neutralized immediately after elution, followed by dialysis against PBS.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab4897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/400.
IHC-P 1/50 - 1/100.
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 101 kDa.
IHC-Fr 1/100.

Target

  • FunctionRequired for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
  • Tissue specificityHighly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
  • Sequence similaritiesBelongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • DomainThe PWWP domain is essential for targeting to pericentric heterochromatin.
  • Post-translational
    modifications
    Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
  • Cellular localizationNucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.
  • Information by UniProt
  • Database links
  • Alternative names
    • DNA (cytosine 5 ) methyltransferase 3 alpha antibody
    • DNA (cytosine 5) methyltransferase 3 alpha antibody
    • DNA (cytosine 5) methyltransferase 3A antibody
    • DNA (cytosine-5)-methyltransferase 3A antibody
    • DNA cytosine methyltransferase 3A2 antibody
    • DNA methyltransferase 3a antibody
    • DNA methyltransferase HsaIIIA antibody
    • DNA MTase HsaI antibody
    • DNA MTase HsaIIIA antibody
    • DNM3A_HUMAN antibody
    • DNMT 3a antibody
    • DNMT antibody
    • DNMT antibody
    • Dnmt3a antibody
    • DNMT3A2 antibody
    • M HsaIIIA antibody
    • M.HsaIIIA antibody
    • MCMT antibody
    • Methyl CpG binding domain protein 3a antibody
    • OTTHUMP00000201149 antibody
    see all

Anti-Dnmt3a antibody images

  • ab4897 staining Dnmt3a in human hepatocarcinoma (HC) tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
  • Anti-Dnmt3a antibody (ab4897) + T247-D cell lysate (Top)

    Secondary
    HRP-conjugated anti-rabbit

    Observed band size : 101 kDa (why is the actual band size different from the predicted?)

References for Anti-Dnmt3a antibody (ab4897)

This product has been referenced in:
  • Elabd C  et al. DNA methyltransferase-3-dependent nonrandom template segregation in differentiating embryonic stem cells. J Cell Biol 203:73-85 (2013). Read more (PubMed: 24127215) »
  • Chik F & Szyf M Effects of specific DNMT gene depletion on cancer cell transformation and breast cancer cell invasion; toward selective DNMT inhibitors. Carcinogenesis 32:224-32 (2011). WB ; Human . Read more (PubMed: 20980350) »

See all 2 Publications for this product

Product Wall

Thank you for your phone call.
I am sorry that the antibody is not working as expected. As we discussed, I have arranged for a free of charge replacement with ab4897. Please see details below. The item is currently backordered, but the estimated de...

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Thank you very much for your interest in ab4897.

To our knowledge, ab4897 has not been tested in Mouse and Rat.It is very likely that ab4897works in these species since thehomolgy between the immunogen peptide and the proteins of interest is 1...

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Thank you for your reply.
I am sorry to confirm it is not possible to provide a blocking peptide on this occasion. I apologise for the inconvenience, I understand this would have been useful in this case.

I hope the other suggestions pr...

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Thank you for taking the time to contact us. I am sorry to hear you have had difficulty obtaining satisfactory results from these antibody.

I would like to reassure you that these antibodies are tested and covered by our 6 month guarantee fo...

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Application Immunoprecipitation
Sample Human Cell lysate - whole cell (lung carcinoma)
Total protein in input 300 µg
Specification lung carcinoma
Immuno-precipitation step Protein G
Username

Abcam user community

Verified customer

Submitted Jan 19 2011

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (hESC, mESC)
Specification hESC, mESC
Fixative Paraformaldehyde
Permeabilization Yes - 0.1% Triton X-100 in PBS for 15min at room temp.
Blocking step BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 21°C
Username

Abcam user community

Verified customer

Submitted Sep 11 2007

The originator used T47-D cell lysate as a positive control for ab4897 in Western blotting. If you have any more questions, please let us know.

To our knowledge, this antibody has yet to be tested in this application. All tested applications are specified on Abcam product datasheets. If you decide to go ahead and purchase this product, please let us know how you get on and in return we will fo...

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The antibody has not been tested for its reactivity with either of these therefore we have no further information on this.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"