You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Dnmt3a protein (Tagged-His Tag) (ab92240)

Submit an Abreview Q&A (1)

Overview

  • Product nameDnmt3a protein (Tagged-His Tag)See all Dnmt3a proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 269-546 (278 amino acids) of Human Dnmt3a (Isoform b) with N terminal His tag; MWt 32kDa (NP_715640).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitution volume is batch dependent.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesRKSTAEKPKVKEIIDERTRERLVYEVRQKCRNIEDICISC GSLNVTLEH PLFVGGMCQNCKNCFLECAYQYDDDGYQS YCTICCGGREVLMCGNNNCC RCFCVECVDLLVGPGAAQ AAIKEDPWNCYMCGHKGTYGLLRRREDWPSR LQMFFAN NHDQEFDPPKVYPPVPAEKRKPIRVLSLFDGIATGLLVLK DL GIQVDRYIASEVCEDSITVGMVRHQGKIMYVGDVRS VTQKHIQEWGPFD LVIGGSPCNDLSIVNPARKGLYEGT GRLFFEFY
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92240 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        DNA (cytosine 5) methyltransferase 3 alphaDNA (cytosine 5) methyltransferase 3ADNA (cytosine-5)-methyltransferase 3A
        DNA cytosine methyltransferase 3A2DNA methyltransferase 3aDNA methyltransferase HsaIIIADNA MTase HsaIIIADNM3A_HUMANDNMTDNMT 3aDnmt3aDNMT3A2M.HsaIIIAMCMTOTTHUMP00000201149
      see all
  • FunctionRequired for genome wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZNF238. Can actively repress transcription through the recruitment of HDAC activity.
  • Tissue specificityHighly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung.
  • Sequence similaritiesBelongs to the C5-methyltransferase family.
    Contains 1 ADD domain.
    Contains 1 GATA-type zinc finger.
    Contains 1 PHD-type zinc finger.
    Contains 1 PWWP domain.
  • DomainThe PWWP domain is essential for targeting to pericentric heterochromatin.
  • Post-translational
    modifications    Sumoylated; sumoylation disrupts the ability to interact with histone deacetylases (HDAC1 and HDAC2) and repress transcription.
  • Cellular localizationNucleus. Cytoplasm. Accumulates in the major satellite repeats at pericentric heterochromatin.

    • Target information above from: UniProt accession Q9Y6K1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Dnmt3a protein (Tagged-His Tag) images

    • SDS-PAGE - Dnmt3a protein (Tagged-His Tag) (ab92240)
      SDS-PAGE - Dnmt3a protein (Tagged-His Tag) (ab92240)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for Dnmt3a protein (Tagged-His Tag) (ab92240)

    ab92240 has not yet been referenced specifically in any publications.

    Publishing research using ab92240 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab92240 Submit a Question for ab92240

    Displaying 1 - 1 of 1 results for Abreviews and Q&A

    10
    Votes: Highest First

    Can you tell me the reconstitution volume and concentration for this product?

    Thank you for your enquiry.

    Here are the reconstitution volume with aqua dest. and final concentration for our current batches.


    1) Lot E1208A05-891
    Reconstitution volume= 35ul
    Concentration = 312 mg/ml

    2) Lo...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"