Products:Epigenetics and Nuclear Signaling >> DNA methylation >> DNMTs
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ab46666 |
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ab2851 has been referenced in 15 publications.
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ICC/IF image of ab2851 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2851, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
All lanes : Anti-Dnmt3b antibody - ChIP Grade (ab2851) at 1 µg/ml
Lane 1 : A498 (Human Kidney Carcinoma) Whole Cell Lysate
Lane 2 : JEG-3 (Human placental choriocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 97.5 kDa
Observed band size : 97.5 kDa
Additional bands at : 32 kDa,46 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 2 minutes
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