Donkey F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed (ab98766)

Overview

• Product nameDonkey F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbedSee all IgG secondary antibodies ...
• Target speciesMouse
• Tested applicationsFlow Cyt, ICC/IF, IHC-P more details
• Minimal
  cross-reactivity
  Chicken, Cow, Goat, Horse, Human, Rabbit, Rat, Sheep
  To ensure minimal cross-reactivity, the antibody has been pre-adsorbed with serum proteins from the following species.
  more details

Properties

• FormLiquid
• Storage instructionsStore at +4°C in the dark.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.2% BSA, PBS
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 488.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Secondary antibodies
  • anti-Mouse
  • IgG
  • Fluorophore
  • DyLight® 488

  • Immunology
  • Immunoglobulins
  • Heavy Chain
  • IgG

Applications

Donkey F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed images

• 

  Immunohistochemistry (Frozen sections) - Donkey F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed (ab98766)Courtesy of Dr. Shaohua Li, UMDNJ-Robert Wood Johnson Medical School

  Sample: mouse embryonic stem cell-differentiated embryoid bodies (EBs)
  
  Preparation:

  • Fix in 3%PFA in PBS for 30 min at RT
  • Incubate in 7.5% sucrose-PBS for 3h at RT
  • Incubate in 15% sucrose-PBS at 4 degree Celsius overnight
  • Embed the EBs in tissue-Tek OCT compound
  • Cut frozen sections to 4-20 µm thickness

  
  
  Primary antibodies:
  Mouse anti-disabled-2 (endoderm marker), 1:100
  Rabbit anti-lamin a 1 (basement membrane marker), 1:400
  
  Secondary antibodies:
  Donkey F(ab')2 polyclonal Secondary Antibody to Mouse IgG -H&L (DyLight® 488), pre-adsorbed (ab98766), 1:200
  Goat anti-rabbit IgG Cy3®, 1:200
  
  Nuclei were counterstained with DAPI
• 

  DyLight® - Donkey F(ab')2 polyclonal Secondary Antibody to Mouse IgG - H&L (DyLight® 488), pre-adsorbed (ab98766)
  Emission spectra of DyLight® fluorochromes available in our catalog.
  Line colors represent the approximate visible colors of the wavelength maxima.