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Donkey anti-Rabbit IgG H&L (DyLight® 550) secondary antibody (ab98489)

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Overview

  • Product nameDonkey anti-Rabbit IgG H&L (DyLight® 550) secondary antibodySee all IgG secondary antibodies ...
  • Description
    Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed
  • Target speciesRabbit
  • Tested applicationsFlow Cyt, ICC/IF, IHC-P more details
  • Minimal
    cross-reactivity
    Chicken, Cow, Goat, Horse, Human, Mouse, Rat, Sheep more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads. F(ab')2 fragment were generated using a pepsin digestion. Fc fragments and whole IgG molecules have been removed. Fragments were conjugated to DyLight® 550.
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesDyLight® 550 replaces DyLight® 549.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab98489 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Flow Cyt: 1/50 - 1/200.
ICC/IF ICC/IF: 1/50 - 1/500.
IHC-P IHC-P: 1/50 - 1/500. DyLight®550 replaces DyLight®549. The DyLight®550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight��549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.

Donkey anti-Rabbit IgG H&L (DyLight® 550) secondary antibody images

  • DyLight®-Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed(ab98489)
    DyLight®-Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 550), pre-adsorbed(ab98489)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Donkey anti-Rabbit IgG H&L (DyLight® 550) secondary antibody (ab98489)

ab98489 has not yet been referenced specifically in any publications.

Publishing research using ab98489 ? Please let us know so that we can cite the reference in this datasheet

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Votes: Highest First

I am using this secondary and getting a very speckly pattern on my slides. The primary is working fine and I have not had this problem with multiple other secondaries I have tested. Can I receive a replacement?

Thank you for contacting Abcam regarding ab98489.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"