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Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed (ab7007)

Submit an Abreview Q&A (4)Specific References (5)

Overview

  • Product nameDonkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbedSee all IgG secondary antibodies ...
  • Target speciesRabbit
  • Tested applicationsIHC-P, ICC/IF, Immunomicroscopy, Flow Cyt more details
  • Minimal
    cross-reactivity
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rat, Sheep more details
  • Immunogen

    Rabbit IgG whole molecule

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage bufferPreservative: None, 0.05% Sodium Azide, 0.01% Thimerosal (merthiolate)
    Constituents: 15mg/ml BSA, 0.25M Sodium chloride, 0.01M Sodium phosphate, pH 7.6
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPrepared from monospecific antiserum by immunoaffinity chromatography using rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Minimum Cross Reactivity to Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat & Sheep Serum Proteins
  • Conjugation notesR-Phycoerythrin (R-PE) Molecular Weight 240,000 daltons) Absorption Wavelength: 488 nm Emission Wavelength: 575 nm OD566nm/OD280nm: 2.8
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab7007 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/200.
ICC/IF ICC/IF: 1/100 - 1/250.
Immunomicroscopy IM: Use at an assay dependent dilution.
Flow Cyt Flow Cyt: 1/100 - 1/250. The maximum amount of reagent required to stain 1 x 106 cells in flow cytometry is approximately 1.0 ug of antibody conjugate.

Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed images

  • Flow Cytometry - Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed (ab7007)
    Flow Cytometry - Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed (ab7007)
    Ab7007 was used with the primary antibody ab18203 in Flow Cyt. See Abreview on ab18203.
  • Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed (ab7007)
    Immunocytochemistry/ Immunofluorescence - Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed (ab7007)
    Ab7007 was used at dilution 1/250 with the primary antibody ab24182 in ICC/IF. See the review on ab24182.

References for Donkey F(ab')2 polyclonal Secondary Antibody to Rabbit IgG - H&L (PE), pre-adsorbed (ab7007)

This product has been referenced in:
  • Kästle M  et al. Combination of PDT and inhibitor treatment affects melanoma cells and spares keratinocytes. Free Radic Biol Med 50:305-12 (2011). Flow Cyt . Read more (PubMed: 21078385) »
  • Jones CI  et al. A functional genomics approach reveals novel quantitative trait loci associated with platelet signaling pathways. Blood 114:1405-16 (2009). Flow Cyt . Read more (PubMed: 19429868) »

See all 5 Publications for this product

Publishing research using ab7007 ? Please let us know so that we can cite the reference in this datasheet

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Customer kindly contacted us to inquire about the immunogen sequence for ab56215.

Thank you for contacting us today regarding secondary antibodies.

I have made a short list of compatible antibodies for use withab56215 Anti-SLC4A4 antibody.These products are pre-absorbed to ensure minimum cross reactivity. Each product is con...

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High FACS signal from secondary-only control. What modification do you suggest?

Thank you for your reply. Your protocol looks correct. The only possible issues I see are the concentration of the secondary antibody that you incubate with the cells but more importantly, the lack of saponin in the wash buffer. The saponin permeabilizati...

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Dear Sirt’s Reference to the above subject (Glucocorticoid Receptor; ab9568 or ab109022) .. the customer need to work it by Flow Cytometer.. and I think this type of work need a secondary anti-body. But the TDS (technical data sheet) on the abcam website ...

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Thank you for your enquiry and interest in our products. In order to be able to search the compatible secondary antibodies, first  it is important to consider the isotypes of these two primary antibodies: ab9568 - mouse IgG1 kappa ab109022 - rabbi...

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Please confirm that this antibody has been tested for application in IHC-frozen sections, and if yes, what dilution is recommended.

Thank you for your phone call. I checked with the originator of this antibody and they informed me that it was not specifically tested for application in IHC-frozen sections or formalin-fixed paraffin embedded sections. I have updated our datasheet to r...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"