Donkey Anti-Rabbit IgG H&L (DyLight® 650) (ab96894)
Key features and details
- Donkey Anti-Rabbit IgG H&L (DyLight® 650)
- Conjugation: DyLight® 650. Ex: 654nm, Em: 673nm
- Host species: Donkey
- Isotype: IgG
- Suitable for: Flow Cyt, ICC/IF, IHC-P
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (DyLight® 650)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. -
Tested applications
Suitable for: Flow Cyt, ICC/IF, IHC-Pmore details -
Conjugation
DyLight® 650. Ex: 654nm, Em: 673nm
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.2% BSA, PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650. -
Clonality
Polyclonal -
Isotype
IgG -
General notes
DyLight® 650 replaces DyLight® 649. -
Research areas
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab96894 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/50 - 1/200.
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ICC/IF |
1/50 - 1/500.
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IHC-P |
1/50 - 1/500.
DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
Notes |
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Flow Cyt
1/50 - 1/200. |
ICC/IF
1/50 - 1/500. |
IHC-P
1/50 - 1/500. DyLight® 650 replaces DyLight® 649. The DyLight® 650 excitation is 652nm and the emission is 672nm. DyLight® 650 hence provides the same far-red fluorescence and photostability as the DyLight® 649 dye. Please refer to the vial in order to check whether the product you received is DyLight® 649 or DyLight® 650. This information however will have no impact on your experiments. For more information please refer to DyLight® 650. |
Images
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (7)
ab96894 has been referenced in 7 publications.
- Shi J et al. Exploration and verification of COVID-19-related hub genes in liver physiological and pathological regeneration. Front Bioeng Biotechnol 11:1135997 (2023). PubMed: 36911196
- Zhang XH et al. Targeting the non-ATP-binding pocket of the MAP kinase p38? mediates a novel mechanism of cytotoxicity in cutaneous T-cell lymphoma (CTCL). FEBS Lett 595:2570-2592 (2021). PubMed: 34455585
- Wakasugi T et al. Role of smooth muscle cell p53 in pulmonary arterial hypertension. PLoS One 14:e0212889 (2019). PubMed: 30807606
- Gosselin EA et al. Multi-layered silk film coculture system for human corneal epithelial and stromal stem cells. J Tissue Eng Regen Med 12:285-295 (2018). PubMed: 28600807
- Tschurtschenthaler M et al. Defective ATG16L1-mediated removal of IRE1a drives Crohn's disease-like ileitis. J Exp Med 214:401-422 (2017). PubMed: 28082357
- Ebisawa M et al. Significance of caveolin-1 and matrix metalloproteinase 14 gene expression in canine mammary tumours. Vet J N/A:N/A (2015). IF . PubMed: 26364240
- Goggolidou P et al. ATMIN is a transcriptional regulator of both lung morphogenesis and ciliogenesis. Development 141:3966-77 (2014). PubMed: 25294941