Donkey Anti-Rabbit IgG H&L (HRP) (ab16284)
Key features and details
- Donkey Anti-Rabbit IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB
Overview
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Product name
Donkey Anti-Rabbit IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rabbit -
Specificity
Reacts with the heavy and light chains of the immunoglobulin molecule. -
Tested applications
Suitable for: WBmore details -
Immunogen
Synthetic peptide corresponding to IgG.
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Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Polyclonal -
Isotype
IgG -
General notes
Part of the AbExcel range. -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16284 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/2000 - 1/20000. Optimized for ECL applications.
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Notes |
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WB
1/2000 - 1/20000. Optimized for ECL applications. |
Images
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All lanes : Anti-beta Actin antibody - Loading Control (ab8227) at 1 µg/ml
Lanes 1-6 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.
Secondary
Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/2000 dilution
Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/10000 dilution
Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab16284) at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time : 150 seconds
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (66)
ab16284 has been referenced in 66 publications.
- Thejer BM et al. Sigma-2 Receptor Ligand Binding Modulates Association between TSPO and TMEM97. Int J Mol Sci 24:N/A (2023). PubMed: 37047353
- Papandreou ME et al. Nucleophagy delays aging and preserves germline immortality. Nat Aging 3:34-46 (2023). PubMed: 37118512
- Shi Y et al. The Antiviral Factor SERINC5 Impairs the Expression of Non-Self-DNA. Viruses 15:N/A (2023). PubMed: 37766367
- Ruivo CF et al. Extracellular Vesicles from Pancreatic Cancer Stem Cells Lead an Intratumor Communication Network (EVNet) to fuel tumour progression. Gut N/A:N/A (2022). PubMed: 35012996
- Niu F et al. The m6A reader YTHDF2 is a negative regulator for dendrite development and maintenance of retinal ganglion cells. Elife 11:N/A (2022). PubMed: 35179492