Donkey Anti-Rat IgG H&L (HRP) (ab102182)
Key features and details
- Donkey Anti-Rat IgG H&L (HRP)
- Conjugation: HRP
- Host species: Donkey
- Isotype: IgG
- Suitable for: WB, ELISA, ICC, IHC-P
Overview
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Product name
Donkey Anti-Rat IgG H&L (HRP)
See all IgG secondary antibodies -
Host species
Donkey -
Target species
Rat -
Specificity
By immunoelectrophoresis and ELISA this antibody reacts specifically with rat IgG and with light chains common to other rat immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins. This antibody may cross react with IgG from other species. -
Tested applications
Suitable for: WB, ELISA, ICC, IHC-Pmore details -
Conjugation
HRP
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 6.8
Constituents: 0.2% BSA, 0.05% CMIT/MIT based preservative -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Substrate reagent
- TMB ELISA Substrate (Highest Sensitivity) (ab171522)
- TMB ELISA Substrate (High Sensitivity) (ab171523)
- TMB ELISA Substrate (Fast Kinetic Rate) (ab171524)
- TMB ELISA Substrate (Slow Kinetic Rate) (ab171525)
- TMB ELISA Substrate (Slower Kinetic Rate) (ab171526)
- TMB ELISA Substrate (Slowest Kinetic Rate) (ab171527)
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab102182 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
WB |
1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminescence: 1/10000 - 1/50000).
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ELISA |
1/10000 - 1/100000. (Primary).
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ICC |
1/200 - 1/5000.
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IHC-P |
1/200 - 1/5000.
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Notes |
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WB
1/5000 - 1/50000. (Colorimetric: 1/5000 - 1/30000; Chemiluminescence: 1/10000 - 1/50000). |
ELISA
1/10000 - 1/100000. (Primary). |
ICC
1/200 - 1/5000. |
IHC-P
1/200 - 1/5000. |
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab102182 has been referenced in 7 publications.
- Ruiz-Velasco A et al. Restored autophagy is protective against PAK3-induced cardiac dysfunction. iScience 26:106970 (2023). PubMed: 37324527
- Kaur N et al. Paracrine signal emanating from stressed cardiomyocytes aggravates inflammatory microenvironment in diabetic cardiomyopathy. iScience 25:103973 (2022). PubMed: 35281739
- Cui H et al. Prolyl oligopeptidase acts as a link between chaperone-mediated autophagy and macroautophagy. Biochem Pharmacol 197:114899 (2022). PubMed: 34968496
- Li R et al. An Antagonistic Peptide of Gpr1 Ameliorates LPS-Induced Depression through the Hypothalamic-Pituitary-Ovarian Axis. Biomolecules 11:N/A (2021). PubMed: 34207497
- Chen Z et al. R-spondin3 promotes the tumor growth of choriocarcinoma JEG-3 cells. Am J Physiol Cell Physiol 318:C664-C674 (2020). PubMed: 31851527
- Kashiwagi M et al. Widely Distributed Neurotensinergic Neurons in the Brainstem Regulate NREM Sleep in Mice. Curr Biol 30:1002-1010.e4 (2020). PubMed: 32032507
- Cao A et al. Galectin-9 binds IgM-BCR to regulate B cell signaling. Nat Commun 9:3288 (2018). PubMed: 30120235