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Donkey polyclonal Secondary Antibody to Goat IgG - H&L (6nm Gold), pre-adsorbed (ab105268)

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Overview

  • Product nameDonkey polyclonal Secondary Antibody to Goat IgG - H&L (6nm Gold), pre-adsorbedSee all IgG secondary antibodies ...
  • Target speciesGoat
  • SpecificityRecognizes the heavy chains on goat IgG and the light chains common to most goat immunoglobulins. Does not crossreact with non-immunoglobulin serum proteins. Exhibits minimal crossreactivity to chicken, guinea pig, hamster, horse, human, mouse, rabbit, rat serum proteins.
  • Tested applicationsELISA, Electron Microscopy, WB more details
  • Minimal
    cross-reactivity
    Chicken, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Rat more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 15mg/ml BSA, 0.15M Sodium chloride, 10mM Sodium phosphate, 10mM Sodium borate, pH 8.5
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab105268 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: Use at an assay dependent dilution.
Electron Microscopy EM: Use at an assay dependent concentration.
WB WB: Use at an assay dependent dilution.

References for Donkey polyclonal Secondary Antibody to Goat IgG - H&L (6nm Gold), pre-adsorbed (ab105268)

ab105268 has not yet been referenced specifically in any publications.

Publishing research using ab105268 ? Please let us know so that we can cite the reference in this datasheet

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Thank you for your quick reply. However, it is surprising that a 1:5,000 dilution will work given that we typically use such high dilutions only for enzyme conjugates for Western blots. Typically, gold-conjugated antibodies are used in the 1:50 range. Was...

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Thank you for your reply.
I am sorry for the confusion about the concentration for this antibody and the recommended starting concentrations to use.
Even though the concentration of 0.1ug/ml is no longer valid in this case, we still guarantee th...

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I just received donkey anti-goat (6 nm gold) – cat #ab105268 and the recommended dilution for EM is 0.1 ug/ml. However, there is no information in the insert or on the tube as to what concentration the solution is. Please let me know the concentrati...

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Thank you for contacting Abcam.
Unfortunately we do not know the antibody concentration in gold complexes because we separate monomers from multimers on density gradients. We do not have any reliable way to measure the antibody concentration on the m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"