Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107)

Overview

• Product nameDonkey polyclonal Secondary Antibody to Goat IgG - H&L (AP)See all IgG secondary antibodies ...
• Target speciesGoat
• SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
• Tested applicationsICC, IHC-P, ELISA, WB more details

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.2% BSA, 0.1M Sodium chloride, 50mM HEPES, 1mM Magnesium chloride, 0.1mM Zinc chloride, pH 7.1
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Alkaline Phosphatase (AP).
• Conjugation notesMolar enzyme: antibody protein ratio is 1:1
• Clonality Polyclonal
• IsotypeIgG
• General notesPart of the AbExcel range.
• Research Areas
  • Secondary antibodies
  • anti-Goat
  • IgG
  • Enzyme
  • Alkaline Phos

  • Immunology
  • Immunoglobulins
  • Heavy Chain
  • IgG

Applications

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) images

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  Western blot - Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107)

  All lanes : Anti-beta Tubulin antibody - Loading Control (ab21057) at 1 µg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane. Secondary Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/5000 dilution Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/20000 dilution Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (AP) (ab97107) at 1/50000 dilution Developed using the ECL technique. Performed under reducing conditions. Exposure time : 8 minutes