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Donkey anti-Goat IgG H&L (DyLight® 650) secondary antibody (ab96938)

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Overview

  • Product nameDonkey anti-Goat IgG H&L (DyLight® 650) secondary antibodySee all IgG secondary antibodies ...
  • Description
    Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 650), pre-adsorbed
  • Target speciesGoat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to chicken, horse, human, mouse, pig, rabbit and rat was detected.
  • Tested applicationsIHC-P, ICC/IF, Flow Cyt more details
  • Minimal
    cross-reactivity
    Chicken, Horse, Human, Mouse, Pig, Rabbit, Rat more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using chicken, human, mouse, pig, rabbit and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesDyLight® 650 replaces DyLight® 649.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab96938 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650.
ICC/IF ICC/IF: 1/50 - 1/500.
Flow Cyt Flow Cyt: 1/50 - 1/200.

Donkey anti-Goat IgG H&L (DyLight® 650) secondary antibody images

  • DyLight®-Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 650), pre-adsorbed(ab96938)
    DyLight®-Donkey polyclonal Secondary Antibody to Goat IgG - H&L (DyLight® 650), pre-adsorbed(ab96938)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Donkey anti-Goat IgG H&L (DyLight® 650) secondary antibody (ab96938)

ab96938 has not yet been referenced specifically in any publications.

Publishing research using ab96938 ? Please let us know so that we can cite the reference in this datasheet

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Customer inquired about detecting presence of 7 different proteins in brain tissue via IHC-F. Would like 3-4 different host species for primary, secondary antibodies that do not overlap in spectra. Customer is also not sure if Caspase 3, Nogo, and neurofi...

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I managed to find antibodies against rat GFAP, FOX3, Iba1, and CD68 all raised in different hosts (chicken, rabbit, goat, and mouse, respectively). They each are highly rated by other researchers and have numerous ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"