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AbExcel

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97110)

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Overview

  • Product nameDonkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP)See all IgG secondary antibodies ...
  • Target speciesGoat
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Goat IgG and with light chains common to other Goat immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsIHC-P, ELISA, WB, ICC more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.1% Proclin
    Constituent: PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesPart of the AbExcel range.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab97110 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/200 - 1/500.
ELISA ELISA: 1/10000 - 1/100000. (Primary).
WB WB: 1/1000 - 1/30000. Colorimetric: 1/1000 - 1/10000. Chemiluminescent: 1/1000 - 1/30000. In some cases the antibody may be diluted further than indicated.
ICC ICC: 1/200 - 1/500.

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) images

  • Western blot - Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97110)
    Western blot - Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97110)
    All lanes : Anti-beta Actin antibody (ab8229) at 1 µg/ml and HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg per lane.

    Secondary
    Lanes 1 - 2 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97110) at 1/5000 dilution
    Lanes 3 - 4 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97110) at 1/10000 dilution
    Lanes 5 - 6 : Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97110) at 1/20000 dilution

    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 20 minutes

References for Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab97110)

ab97110 has not yet been referenced specifically in any publications.

Publishing research using ab97110 ? Please let us know so that we can cite the reference in this datasheet

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I have been using ab97110 as a secondary for my Westerns. However, since we only need such a amount and very dilute, we end up wasting a lot of it. I only am running Westerns semi-regularly and the workload comes in waves, so it possible to aliquot and fr...

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We have studied the effect of freeze thaw cycles on conjugated antibodies and have seen that they can have significant effect on HRP secondaries. As such we do not recommend stroage at -20C. These products should have good activity for one year or more...

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Sir/ Madam, Sorry to disturb you. Our customer makes a request about ab97110:Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (lot:gr13665-10). He has ordered this antibody recently, and he would like to get the COC. Would you please help him ...

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Thank you for your enquiry. Please find enclosed as requested the CoC for ab97110, lot number GR13665-10. I hope this information is helpful to you. Please do not hesitate to contact us if you need any additional information or advice.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"