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Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6885)

Submit an Abreview Q&A (6)Specific References (9)

Overview

  • Product nameDonkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP)See all IgG secondary antibodies ...
  • Target speciesGoat
  • Tested applicationsDot Blot, ELISA, IHC-P, IHC-Fr, Immunomicroscopy, WB, ICC/IF more details
  • Immunogen

    Full length native Goat IgG (purified).

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesHorseradish Peroxidase (HRP)
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6885 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Dot Blot Dot: Use at an assay dependent dilution.
ELISA ELISA: 1/300000.
IHC-P IHC-P: Use at an assay dependent dilution.
IHC-Fr IHC-Fr: Use at an assay dependent dilution.
Immunomicroscopy IM: Use at an assay dependent dilution.
WB WB: Use at an assay dependent dilution.
ICC/IF ICC/IF: 1/1000 - 1/2500.

Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) images

  • Western blot - Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6885)
    Western blot - Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6885)
    Ab6885 was used at dilution 1/5000 with the primary antibody ab36146 in WB. See the review on ab36146.

References for Donkey polyclonal Secondary Antibody to Goat IgG - H&L (HRP) (ab6885)

This product has been referenced in:
  • Goel M  et al. Cochlin Induced TREK-1 Co-Expression and Annexin A2 Secretion: Role in Trabecular Meshwork Cell Elongation and Motility. PLoS One 6:e23070 (2011). WB . Read more (PubMed: 21886777) »
  • Kim B  et al. Bone morphogenetic protein-1 processes insulin-like growth factor-binding protein 3. J Biol Chem 286:29014-25 (2011). Read more (PubMed: 21697095) »

See all 9 Publications for this product

Publishing research using ab6885 ? Please let us know so that we can cite the reference in this datasheet

Product Wall

Submit an Abreview for ab6885 Submit a Question for ab6885

Displaying 1 - 6 of 6 results for Abreviews and Q&A

10
Votes: Highest First

Dear Sir/ Madam,
I am looking for a peroxidase secondary antibody to reacts with goat. The antibody that I am using is ab 13627. In your website I found ab 6741, ab 6885 and ab 97100. Could you please indicate which one is better to react with ab1362...

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Thank you for contacting us.

As I presume these antibodies will be used in ICC/IF (which is the application ab13627 has been tested in), I would then suggest using ab6885 or ab6741 as secondary antibody.

Both should recognise and be su...

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I convinced them to accept the replacements. I believe they will not have problems with the new secondaries, but please make sure the replacements do not have brown precipitates in the vials.

I am looking forward to hearing from you.

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I have requested that you be sent 3 new antibodies for the customer and I have also asked that the vials be checked for brown precipitate before being shipped.

Thank you for all your help with this matter and I hope it all works out.

P...

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I have some bad news. Please, read below what customer answered me about this case:

I would like to inform you that the antibodies were tested in WB experiments and the incubation time and concentrations used are the same that work for other se...

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I am really sorry that the customer is so upset, that was never my intention. I understand that they are well versed in the WB field; howeverI was only trying to get some more information on the protocol that they had used.

If the customers are ...

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\Our customer is still complaining that all these 3 secondaries are not working. The group has experience inWBapplication as this is the major techniqueperformed in their lab.Unfortunatelly, they are very disapointed with these secondaries they received.

Thank you for contacting Abcam.

I am sorry that your customer is still having problems with these antibodies. As I mentioned in my previous email in November, could you provide me with the protocol that they are using and I can see if there is a...

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For the technical support,   Customer has reported brown precipitates in the vials below, and after testing none of them worked. I suggested vortexing the vials as recommended on the datasheet, but nothing changed, customer says the antibodies are not wor...

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I am sorry that you are experiencing problems with the HRP tagged antibodies that you purchased from us. As you mentioned in the email that was sent, it is common for hrp tagged secondaries to have a brown precipitate in the vial, but vortexing should all...

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Hej ! What's the optimal dilution for this ab ? (8. Dilute the horseradish peroxidase labeled secondary antibody at the optimal dilution (according to the manufacturer) in blocking buffer.)

We recommend the follwing dilutions: ELISA 1:4,000 - 1:10,000 Immunoblotting 1:2,000 - 1:5,000 Immunohistochemistry 1:200 - 1:2,000

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"