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Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6820)

Submit an Abreview Q&A (7)Specific References (1)

Overview

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesHorseradish Peroxidase (HRP)
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6820 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Dot Blot Dot: Use at an assay dependent concentration.
ELISA ELISA: 1/70000.
IHC-P IHC-P: 1/500 - 1/2000.
IHC-Fr IHC-Fr: 1/500 - 1/2000.
WB WB: 1/2000 - 1/10000.
ICC/IF ICC/IF: 1/500 - 1/2500.

References for Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6820)

This product has been referenced in:

See 1 Publication for this product

Publishing research using ab6820 ? Please let us know so that we can cite the reference in this datasheet

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Submit an Abreview for ab6820 Submit a Question for ab6820

Displaying 1 - 7 of 7 results for Abreviews and Q&A

10
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Dear xxxxx,

Thank you for your reply.

I am going to address the issues you have stated in your email one by one:

1. I used Avidin/biotin system not to optimise the experiment, but to show that my methodology for IHC was ri...

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Thank you very much for your reply.

I am sorry that the staining using the Donkey polyclonal Secondary Antibody to Mouse IgG (ab6820) has not performed as expected in your experiment. My aim to gather further information relating to the protocol...

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Dear xxxxxx,

Thank you very much for your reply.

I have discussed your reply with my supervisor. She is very concerned that you are asking her to finance (i.e. carry out myself) further analysis using our very valuable human samples....

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Thank you for your reply.

I can understand your concern and my aim is to make sure that we resolve this situation as quickly as possible so that you are able to get on with your research. However, in order to achieve this we need to establish wh...

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Dear Sir/madam,

Thank you for your email. Please find the answers below:

1- No. We do not fix the samples. After obtaining them from the hospital, they are put immediately in OCT and placed in -80 freezer for future use. This is the ...

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Thank you for your very detailed reply and sorry for my late reply.

I have been looking into the problems you have encountered and am still investigating the case. I thinkwith the protocol you have been using that there should be staining. Howev...

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Dear Sir/madam,

With refer to my problem using one of your primary and secondary abs, I was required to fill in the attached document and email it back to you. I would appreciate your concern on this matter.

Kind Regards

Thank you for taking time to completethequestionnaire. The details provided will enableme to investigate this case and will provide us with vital information for monitoring product quality.

Having reviewed the information you have provided ther...

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Phone call reporting staining using the Anti-COX1 / Cyclooxygenase 1 antibody [CYO-1] (ab22696) in combination with Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6820).

Thank you for calling us today and reporting the problem you have beenexperiencing with the Anti-COX1 / Cyclooxygenase 1 antibody [CYO-1] (ab22696)used incombination with the Donkey polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6820). We t...

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What is the recomended dilution factor for ab22696, ab54615, ab103724 and ab126709?

Thank you for contacting us.

we tested catalog ab126709in IP, IHC, ICC, and Flow cytometry and it is tested negative. For this reason, we only recommending the product for western blot.

I hope this information is helpful to you. Please d...

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What is the recomended dilution factor for ab22696, ab54615, ab103724 and ab126709?

Thank you for contacting us.

The ab22696 could be used at 1/10 - 1/100 dilution.
ab54615 at 5-10ug/ml
ab103724 at 5-10ug/ml
ab126709 at 1/500-1/1000 dilution.

ab6820 and ab6802 at 1/500-1/2000 dilution.

I hope this i...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"