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Donkey anti-Rabbit IgG H&L (DyLight® 594) secondary antibody (ab96921)

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Overview

  • Product nameDonkey anti-Rabbit IgG H&L (DyLight® 594) secondary antibodySee all IgG secondary antibodies ...
  • Description
    Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 594), pre-adsorbed
  • Target speciesRabbit
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
  • Tested applicationsFlow Cyt, ICC/IF, IHC-P more details
  • Minimal
    cross-reactivity
    Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesAntiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 594.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab96921 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Flow Cyt Flow Cyt: 1/50 - 1/200.
ICC/IF ICC/IF: 1/50 - 1/500.
IHC-P IHC-P: 1/50 - 1/500.

Donkey anti-Rabbit IgG H&L (DyLight® 594) secondary antibody images

  • DyLight® - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 594), pre-adsorbed (ab96921)
    DyLight® - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 594), pre-adsorbed (ab96921)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.
  • Immunohistochemistry (Frozen sections) - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 594), pre-adsorbed (ab96921)
    Immunohistochemistry (Frozen sections) - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 594), pre-adsorbed (ab96921)

    E14 mouse brain sample was stained with primary antibody Rabbit anti-Clim1. The secondary antibody used was ab96921 (1:200 dilution, incubate for 1 hour at room temperature). The blue background was from DAPI.

    This image is courtesy of an anonymous Abreview

References for Donkey anti-Rabbit IgG H&L (DyLight® 594) secondary antibody (ab96921)

ab96921 has not yet been referenced specifically in any publications.

Publishing research using ab96921 ? Please let us know so that we can cite the reference in this datasheet

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Submit an Abreview for ab96921 Submit a Question for ab96921

Displaying 1 - 6 of 6 results for Abreviews and Q&A

10
Votes: Highest First

Customer inquired about detecting presence of 7 different proteins in brain tissue via IHC-F. Would like 3-4 different host species for primary, secondary antibodies that do not overlap in spectra. Customer is also not sure if Caspase 3, Nogo, and neurofi...

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Thank you for contacting Abcam.

I managed to find antibodies against rat GFAP, FOX3, Iba1, and CD68 all raised in different hosts (chicken, rabbit, goat, and mouse, respectively). They each are highly rated by other researchers and have numerous ...

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Here isPO 310-61664B

Best regards,

Thank you for your email.

The PO-310-61664B was ordered in October 2011 and the complaint was raised in July 2012; this means the product is out of Abpromise guarantee, which is 6 months from date of purchase.

We have sold many units of...

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Hello
Our customer said that primary antibodies are displayed at images (attached again in this email).
In addition, her primary antibodies are raised on goat, so she can not
use Alexa.
Antibodies were stored as recommended (4oC).
Orde...

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Could you please check the purchase order number; the number I-004-61517-br or 004-61517 provided does not match in our system? Could you also provide the purchase date of the product?
I will look forward to hearing from you soon.

Attached below are the questionnaires of our customer.
She is having problems with their secundary antibodies.
Best regards,

Thank you for your email. I am sorry to hear that you have been experiencing problems with this antibody.

I have read the details you have kindly provided and have following further questions for better understanding of the problem;

Coul...

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thank You for this quick and precise answer. You are suggesting to use DyLight 594, but I am not entirely sure that it would be well excited by the 543nm line; moreover, this excitation wavelength is not mentioned in the AbCam fluorochrome guide. From You...

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Thank you for your reply! Indeed, firstly I wanted to suggest also to use Texas Red with TRITC. However when I looked closer at the emission spectra, it seemed to me that there is quite a "bleeding" of the TRITC into the Texas Red. However this depends al...

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Dear AbCam scientific support, I wish to perform a double immunofluorescence staining (i.e. using two different primary antibodies) on brain sections coming from GFP-expressing mice. However, my confocal microscope has only two excitation wavelengths (488...

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Thank you very much for your inquiry. Indeed we do have a number of antibodies which can be suitable for your application. Please find here below a selection. I have only proposed pre-absorbed antibodies, as they will help to reduce background in immunohi...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"