Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 650), pre-adsorbed (ab96922)
Overview
- Product nameDonkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 650), pre-adsorbedSee all IgG secondary antibodies ...
- Target speciesRabbit
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
- Tested applicationsICC/IF, IHC-P, Flow Cyt more details
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Minimal
cross-reactivity
Chicken, Cow, Goat, Horse, Human, Mouse, Pig, Rat more details
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: 0.2% BSA, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesAntiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 650.
- Clonality Polyclonal
- IsotypeIgG
- General notesDyLight® 650 replaces DyLight® 649.
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab96922 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: 1/50 - 1/500. |
| IHC-P | IHC-P: 1/50 - 1/500. DyLight®650 replaces DyLight®649. The DyLight®650 excitation is 652nm and the emission is 672nm. DyLight®650 hence provides the same far-red fluorescence and photostability as the DyLight®649 dye. Please refer to the vial in order to check whether the product you received is DyLight®649 or DyLight®650. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight650. |
| Flow Cyt | Flow Cyt: 1/50 - 1/200. |
Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 650), pre-adsorbed images
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DyLight®-Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 650), pre-adsorbed(ab96922)Emission spectra of DyLight® fluorochromes available in our catalog.
Line colors represent the approximate visible colors of the wavelength maxima.
References for Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (DyLight® 650), pre-adsorbed (ab96922)
ab96922 has not yet been referenced specifically in any publications.
