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Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed (ab98502)

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Overview

  • Product nameDonkey polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbedSee all IgG secondary antibodies ...
  • Target speciesRabbit
  • Tested applicationsIHC-P, ICC/IF, Flow Cyt more details
  • Minimal
    cross-reactivity
    Human, Mouse, Rat more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to FITC.
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab98502 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/50 - 1/500.
ICC/IF ICC/IF: 1/50 - 1/500.
Flow Cyt Flow Cyt: 1/50 - 1/200.

Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed images

  • Immunocytochemistry/ Immunofluorescence - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed (ab98502)
    Immunocytochemistry/ Immunofluorescence - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed (ab98502)Image courtesy of Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
    ab98502 (1/200) detecting ab5176 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (blue); ab5176, a rabbit polyclonal to Histone H3 (phospho S10), was used at 1/4000 dilution.
  • Immunocytochemistry/ Immunofluorescence - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed (ab98502)
    Immunocytochemistry/ Immunofluorescence - Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed (ab98502)Image courtesy of Dr. Kirk McManus, Univ. of Manitoba/Cancer Care MICB, Canada
    ab98502 (1/200) detecting ab5176 in assynchronous HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised in 0.5% Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red); ab5176, a rabbit polyclonal to Histone H3 (phospho S10), was used at 1/4000 dilution.

References for Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (FITC), pre-adsorbed (ab98502)

ab98502 has not yet been referenced specifically in any publications.

Publishing research using ab98502 ? Please let us know so that we can cite the reference in this datasheet

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"