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Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6802)

Submit an Abreview Q&A (3)Specific References (6)

Overview

  • Product nameDonkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)See all IgG secondary antibodies ...
  • Target speciesRabbit
  • Tested applicationsWB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-P, IHC-Fr more details
  • Immunogen

    Rabbit IgG, whole molecule

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesHorseradish Peroxidase (HRP).
  • Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6802 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000 - 1/3000.
ELISA ELISA: 1/160000.
Immunomicroscopy IM: Use at an assay dependent concentration.
Dot Blot Dot: Use at an assay dependent concentration.
ICC/IF ICC/IF: 1/500 - 1/2000.
IHC-P IHC-P: 1/500 - 1/2000.
IHC-Fr IHC-Fr: 1/500 - 1/2000.

References for Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6802)

This product has been referenced in:
  • Pakharukova M  et al. The increased CAR-dependent metabolism of thyroid hormones in mice with high cancer susceptibility. Life Sci 87:439-44 (2010). WB . Read more (PubMed: 20816995) »
  • Wilson JB  et al. FANCG promotes formation of a newly identified protein complex containing BRCA2, FANCD2 and XRCC3. Oncogene 27:3641-52 (2008). WB . Read more (PubMed: 18212739) »

See all 6 Publications for this product

Publishing research using ab6802 ? Please let us know so that we can cite the reference in this datasheet

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What is the recomended dilution factor for ab22696, ab54615, ab103724 and ab126709?

Thank you for contacting us.

we tested catalog ab126709in IP, IHC, ICC, and Flow cytometry and it is tested negative. For this reason, we only recommending the product for western blot.

I hope this information is helpful to you. Please d...

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What is the recomended dilution factor for ab22696, ab54615, ab103724 and ab126709?

Thank you for contacting us.

The ab22696 could be used at 1/10 - 1/100 dilution.
ab54615 at 5-10ug/ml
ab103724 at 5-10ug/ml
ab126709 at 1/500-1/1000 dilution.

ab6820 and ab6802 at 1/500-1/2000 dilution.

I hope this i...

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Hi Abcam,

I tried contacting the abcam distributer here in Australia (Sapphire bioscience), but the representative was unable to answer my questions.

I would like to purchase one secondary antibody against primary abcam antibodies I ...

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Thank you for contacting us.

The secondary antibodies you mentioned might work for dot blots with the rabbit polyclonal Histone primaries, as they are guaranteed to work in Western blot which is quite similar to dot blot. However, we have not te...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"