Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L, pre-adsorbed (ab97081)
Overview
- Product nameDonkey polyclonal Secondary Antibody to Rabbit IgG - H&L, pre-adsorbedSee all IgG secondary antibodies ...
- Target speciesRabbit
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non immunoglobulin serum proteins. Less than 0.1% cross reactivity to bovine, chicken, goat, horse, human, mouse, pig and rat was detected.
- Tested applicationsICC, IHC-P, WB, ELISA more details
-
Minimal
cross-reactivity
Chicken, Cow, Goat, Human, Mouse, Pig, Rat more details
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.09% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesAntiserum was cross adsorbed using bovine, chicken, goat, human, mouse, pig and rat immunosorbents to remove cross reactive antibodies. This antibody was isolated by affinity chromatography using antigen coupled to agarose beads .
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab97081 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC | ICC: Use at an assay dependent dilution. |
| IHC-P | IHC-P: 1/200 - 1/2000. |
| WB | WB: 1/1000 - 1/30000. (Colorimetric: 1/1000 - 1/10000; Chemiluminescent: 1/1000 - 1/30000) |
| ELISA | ELISA: 1/100 - 1/10000. (Coating: 1/100 - 1/500; Primary: 1/1000 - 1/10000) |
References for Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L, pre-adsorbed (ab97081)
ab97081 has not yet been referenced specifically in any publications.
