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Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (6nm Gold), pre-adsorbed (ab105294)

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Overview

  • Product nameDonkey polyclonal Secondary Antibody to Rabbit IgG - H&L (6nm Gold), pre-adsorbedSee all IgG secondary antibodies ...
  • Target speciesRabbit
  • SpecificityRecognizes the heavy chains on rabbit IgG and the light chains common to most rabbit immunoglobulins. Does not crossreact with non-immunoglobulin serum proteins. Exhibits minimal crossreactivity to bovine, chicken, goat, guinea pig, syryan hamster, horse, human, mouse, rat and sheep serum proteins.
  • Tested applicationsElectron Microscopy, WB, ELISA, Other more details
  • Minimal
    cross-reactivity
    Chicken, Cow, Goat, Guinea pig, Horse, Human, Mouse, Rat, Sheep, Syrian Hamster more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage bufferPreservative: 0.05% Sodium Azide
    Constituents: 15mg/ml BSA, 0.15M Sodium chloride, 10mM Sodium phosphate, 10mM Sodium borate, pH 8.5
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab105294 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Electron Microscopy EM: 1/20 - 1/40.
WB WB: Use at an assay dependent dilution.
ELISA ELISA: Use at an assay dependent dilution.
Other Other: Use at an assay dependent dilution. Light microscopy

References for Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (6nm Gold), pre-adsorbed (ab105294)

ab105294 has not yet been referenced specifically in any publications.

Publishing research using ab105294 ? Please let us know so that we can cite the reference in this datasheet

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Submit an Abreview for ab105294 Submit a Question for ab105294

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Do you have an EM protocol for use of the antibody?
When are the primary and secondary antibodies added? Before or after the cells are fixed for EM?

Many thanks

Fixation precedes incubation with primary and secondary antibodies.

The protocol for gold-antibody complexes is essentially the same as for all conjugates with the exception of the buffer used to dilute and wash.


Step 1: Block w...

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Konzentration? Verduennung fuer EM nicht ausrechenbar.

Vielen Dank für Ihren Anruf.

Wie versprochen, habe ich das Labor wegen der genauen Konzentration von ab105294 kontaktiert, und habe erfahren, dass die genaue Konzentration leider nicht bestimmt werden kann. Zwar wird der Antikörper vor der Konj...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"