Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (TR) (ab6800)
Overview
- Product nameDonkey polyclonal Secondary Antibody to Rabbit IgG - H&L (TR)See all IgG secondary antibodies ...
- Target speciesRabbit
- Tested applicationsImmunomicroscopy, Flow Cyt, IHC-P, IHC-Fr, ICC/IF, ELISA more details
- Immunogen
Rabbit IgG, whole molecule
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
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Concentration information loading... - PurityIgG fraction
- Purification notesTexas Red Sulfonyl Chloride (Molecular Weight 625 daltons) Absorption Wavelength: 596 nm Emission Wavelength: 620 nm Fluorochrome/Protein Ratio: 1.7 moles Texas Red per mole of Goat IgG
- Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose
- Clonality Polyclonal
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab6800 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Immunomicroscopy | IM: Use at an assay dependent dilution. |
| Flow Cyt | Flow Cyt: Use at an assay dependent dilution. |
| IHC-P | IHC-P: Use at an assay dependent dilution. |
| IHC-Fr | IHC-Fr: Use at an assay dependent dilution. |
| ICC/IF | ICC/IF: 1/1000 - 1/5000. |
| ELISA | ELISA: 1/10000 - 1/50000. |
References for Donkey polyclonal Secondary Antibody to Rabbit IgG - H&L (TR) (ab6800)
This product has been referenced in:
- Marion V et al. Transient ciliogenesis involving Bardet-Biedl syndrome proteins is a fundamental characteristic of adipogenic differentiation. Proc Natl Acad Sci U S A 106:1820-1825 (2009). ICC/IF . Read more (PubMed: 19190184) »
- Miranda E et al. The intracellular accumulation of polymeric neuroserpin explains the severity of the dementia FENIB. Hum Mol Genet 17:1527-39 (2008). ICC/IF . Read more (PubMed: 18267959) »
