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Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 550) (ab96940)

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Overview

  • Product nameDonkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 550)See all IgG secondary antibodies ...
  • Target speciesSheep
  • SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
  • Tested applicationsIHC-P, ICC/IF, Flow Cyt more details

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to DyLight® 550.
  • Clonality Polyclonal
  • IsotypeIgG
  • General notesDyLight® 550 replaces DyLight® 549.
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab96940 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
IHC-P IHC-P: 1/50 - 1/500. DyLight®550 replaces DyLight®549. The DyLight®550 excitation is 562nm and the emission is 576nm. DyLight®550 provides the same orange-to-red fluorescence and photostability as the DyLight®549 dye. Please refer to the vial in order to check whether the product you received is DyLight®549 or DyLight®550. This information however will have no impact on your experiments. For more information please refer to www.abcam.com/DyLight550.
ICC/IF ICC/IF: 1/50 - 1/500.
Flow Cyt Flow Cyt: 1/50 - 1/200.

Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 550) images

  • DyLight®-Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 550)(ab96940)
    DyLight®-Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 550)(ab96940)
    Emission spectra of DyLight® fluorochromes available in our catalog.
    Line colors represent the approximate visible colors of the wavelength maxima.

References for Donkey polyclonal Secondary Antibody to Sheep IgG - H&L (DyLight® 550) (ab96940)

ab96940 has not yet been referenced specifically in any publications.

Publishing research using ab96940 ? Please let us know so that we can cite the reference in this datasheet

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Hi,

I have reviewed this profile. I might not clarify clearly what I need. I need to have the spectrum of the dye as you may know the excitation spectrum may cover a certain range not only at a certain point. I am doing multiple staining of dif...

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Thank you for your reply. My colleague is not in the office today and that is the reason I am writing to you.

I ma sorry there was a misunderstanding.

We do have information on the spectra of DyLight® fluorochromes on our homepage: <...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"