Donkey anti-Sheep IgG H&L (HRP) secondary antibody (ab97125)
Overview
- Product nameDonkey anti-Sheep IgG H&L (HRP) secondary antibodySee all IgG secondary antibodies ...
- DescriptionDonkey polyclonal Secondary Antibody to Sheep IgG - H&L (HRP)
- Target speciesSheep
- SpecificityBy immunoelectrophoresis and ELISA this antibody reacts specifically with Sheep IgG and with light chains common to other Sheep immunoglobulins. No antibody was detected against non immunoglobulin serum proteins.
- Tested applicationsICC, IHC-P, ELISA, WB more details
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.1% Proclin
Constituents: 0.2% BSA, PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
- Conjugation notesMolar enzyme/ antibody protein ratio is 4:1
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab97125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC | ICC: Use at an assay dependent dilution. |
| IHC-P | IHC-P: 1/200 - 1/5000. |
| ELISA | ELISA: 1/10000 - 1/100000. (primary) |
| WB | WB: 1/5000 - 1/50000. Colorimetric: 1/500 - 1/30000, Chemiluminescent: 1/10000 - 1/50000 |
References for Donkey anti-Sheep IgG H&L (HRP) secondary antibody (ab97125)
ab97125 has not yet been referenced specifically in any publications.
