Anti-Dopamine Receptor D1 antibody (ab20066)

Overview

  • Product nameAnti-Dopamine Receptor D1 antibodySee all Dopamine Receptor D1 primary antibodies ...
  • Description
    Rabbit polyclonal to Dopamine Receptor D1
  • SpecificityStains specific cells in various regions of PLP fixed Rat brain sections. This includes Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area. Cross reactivity: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; D5 Dopamine Receptor 0%
  • Tested applicationsWB, ICC/IF, IHC-FoFr, IHC-P, ELISA more details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide: MDGTGLVVERDFS, corresponding to amino acids 9-21 of Human Dopamine Receptor D1

  • Positive control
    • Whole Rat brain homogenate.

Properties

Applications

Our Abpromise guarantee covers the use of ab20066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa).
ICC/IF Use at an assay dependent concentration. PubMed: 19006818
IHC-FoFr Use at an assay dependent concentration.
IHC-P 1/5000.
ELISA Use at an assay dependent concentration.

Target

  • FunctionDopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
  • Tissue specificityDetected in caudate, nucleus accumbens and in the olfactory tubercle.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • Cellular localizationCell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
  • Target information above from: UniProt accession P21728 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
  • Alternative names
    • D(1A) dopamine receptor antibody
    • D1A dopamine receptor antibody
    • DADR antibody
    • DADR antibody
    • Dopamine D1 receptor antibody
    • Dopamine Receptor D1 antibody
    • DR D1 antibody
    • DR D1A antibody
    • DRD 1 antibody
    • DRD 1A antibody
    • DRD1 antibody
    • DRD1_HUMAN antibody
    • DRD1A antibody
    • DRD1A antibody
    see all

Anti-Dopamine Receptor D1 antibody images

  • ab20066 staining Dopamine Receptor D1 in Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and blocked with 5% serum for 60 minutes at 23°C. The sample was incubated with primary antibody (1/600 in PBS + 5% Serum) at 4°C for 13 hours. An undiluted Alexa Fluor®555-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.

    See Abreview

  • All lanes : Anti-Dopamine Receptor D1 antibody (ab20066) at 1 µg/ml

    Lane 1 : Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
    Lane 2 : Rat Forebrain
    Lane 3 : Rat Cortex Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 49 kDa
    Observed band size : 48 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 45 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 90 seconds

References for Anti-Dopamine Receptor D1 antibody (ab20066)

This product has been referenced in:
  • Paul K  et al. Dampened dopamine-mediated neuromodulation in prefrontal cortex of fragile X mice. J Physiol 591:1133-43 (2013). Mouse . Read more (PubMed: 23148316) »
  • Krishnan B  et al. Dopamine receptor mechanisms mediate corticotropin-releasing factor-induced long-term potentiation in the rat amygdala following cocaine withdrawal. Eur J Neurosci 31:1027-42 (2010). Read more (PubMed: 20377617) »

See all 3 Publications for this product

Product Wall

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Blocking step Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 20°C
Sample Monkey Tissue sections (Brain (Frontal Cortex))
Specification Brain (Frontal Cortex)
Permeabilization Yes - 0.5% Triton X-100
Fixative Formaldehyde
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Submitted Apr 18 2014

Application Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample Mouse Tissue sections (brain)
Specification brain
Fixative Paraformaldehyde
Antigen retrieval step None
Permeabilization No
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

Submitted Apr 24 2013

Thank you for your reply.

We do have a dopamine receptor D1 protein, ab90384, but I didn't recommend it because we haven't tested it as a positive control in WB.

http://www.abcam.com/Dopamine-Receptor-D1-protein-Active-ab90384.html ...

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Thank you for contacting us. I assume you are refering to the ICC/IF application? If not, please let me know. This application was added based on the publication PubMed: 19006818, and has not been tested in house in this application. The publicat...

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Thank you for your inquiry. We would guarantee both ab20066 and ab40653 to work with rat samples in WB in accordance with our Abpromise. http://www.abcam.com/index.html?pageconfig=resource&rid=10372 I wouldn't be too concerned about the linker ...

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Thank you for your telephone call this afternoon. I can confirm that this Dopamine Receptor D1 antibody (ab20066) has been tested in PLP fixed rat brain sections at 1:5,000 dilution. It stains Medial septum, Nucleus accumbens, Dentate gyrus, Globus ...

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