Overview
- Product nameAnti-Dopamine Receptor D1 antibodySee all Dopamine Receptor D1 primary antibodies ...
- DescriptionRabbit polyclonal to Dopamine Receptor D1
- SpecificityStains specific cells in various regions of PLP fixed Rat brain sections. This includes Medial septum, Nucleus accumbens, Dentate gyrus, Globus pallidus, Medial forebrain bundle, Cortex regions 1-3, Substantia Nigra reticulata, and the Ventral tegmental area. Cross reactivity: D1 Dopamine Receptor (9-21) 100%; D1 Dopamine Receptor 90%; D2 Dopamine Receptor (272-282) 0%; D2 Dopamine Receptor 0%; D3 Dopamine Receptor (2-10) 0%; D3 Dopamine Receptor 0; D4 Dopamine Receptor (176-185) 0%; D4 Dopamine Receptor 0%; D5 Dopamine Receptor (23-35) 0%; D5 Dopamine Receptor 0%
- Tested applicationsWB, ICC/IF, IHC-FoFr, IHC-P, ELISA more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide: MDGTGLVVERDFS, corresponding to amino acids 9-21 of Human Dopamine Receptor D1
- Positive controlWhole Rat brain homogenate.
Properties
- FormLiquid
- Storage instructionsStore at +4°C. Aliquot and store at -20°C or -80°C when reconstituted. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: PBS, 10mg/ml BSA - PurityWhole antiserum
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab20066 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/1000. Detects a band of approximately 48 kDa (predicted molecular weight: 49 kDa). |
| ICC/IF | ICC/IF: Use at an assay dependent concentration. PubMed: 19006818 |
| IHC-FoFr | IHC-FoFr: Use at an assay dependent concentration. |
| IHC-P | IHC-P: 1/5000. |
| ELISA | ELISA: Use at an assay dependent concentration. |
Target
- FunctionDopamine receptor whose activity is mediated by G proteins which activate adenylyl cyclase.
- Tissue specificityDetected in caudate, nucleus accumbens and in the olfactory tubercle.
- Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
- Cellular localizationCell membrane. Endoplasmic reticulum membrane. Transport from the endoplasmic reticulum to the cell surface is regulated by interaction with DNAJC14.
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Database links
- Entrez Gene: 1812 Human
- Entrez Gene: 13488 Mouse
- Entrez Gene: 24316 Rat
- Omim: 126449 Human
- SwissProt: P21728 Human
- SwissProt: Q61616 Mouse
- SwissProt: P18901 Rat
- Unigene: 2624 Human
- Unigene: 54161 Mouse
- Unigene: 24039 Rat
see all
Target information above from: UniProt accession
P21728
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- D(1A) dopamine receptor antibodyD1A dopamine receptor antibodyDADR antibody
- DADR antibodyDopamine D1 receptor antibodyDopamine Receptor D1 antibodyDR D1 antibodyDR D1A antibodyDRD 1 antibodyDRD 1A antibodyDRD1 antibodyDRD1_HUMAN antibodyDRD1A antibodyDRD1A antibody
see all
Anti-Dopamine Receptor D1 antibody images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-Dopamine Receptor D1 antibody (ab20066)This image is courtesy of an anonymous Abreviewab20066 staining Dopamine Receptor D1 in Mouse brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Tissue samples were fixed by perfusion with paraformaldehyde and blocked with 5% serum for 60 minutes at 23°C. The sample was incubated with primary antibody (1/600 in PBS + 5% Serum) at 4°C for 13 hours. An undiluted Alexa Fluor®555-conjugated Goat anti-rabbit polyclonal was used as the secondary antibody.
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Western blot - Dopamine Receptor D1 antibody (ab20066)All lanes : Anti-Dopamine Receptor D1 antibody (ab20066) at 1 µg/ml
Lane 1 :Brain (Human) Tissue Lysate - adult normal tissue (ab29466)
Lane 2 : Rat Forebrain
Lane 3 : Rat Cortex Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 49 kDa
Observed band size : 48 kDa (why is the actual band size different from the predicted?)
Additional bands at : 45 kDa,70 kDa,75 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 90 seconds
References for Anti-Dopamine Receptor D1 antibody (ab20066)
This product has been referenced in:
- Paul K et al. Dampened dopamine-mediated neuromodulation in prefrontal cortex of fragile X mice. J Physiol 591:1133-43 (2013). Mouse . Read more (PubMed: 23148316) »
- Krishnan B et al. Dopamine receptor mechanisms mediate corticotropin-releasing factor-induced long-term potentiation in the rat amygdala following cocaine withdrawal. Eur J Neurosci 31:1027-42 (2010). Read more (PubMed: 20377617) »
