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Product Name
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Dopamine Transporter antibody [hDAT-LOOP]
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See all Dopamine Transporter antibodies (6)...
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Product type
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Primary antibodies
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Description
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Rat monoclonal [hDAT-LOOP] to Dopamine Transporter
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Immunogen
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Fusion protein consisting of the second extracellular loop (amino acids 180-218).
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Reacts with
(species key)
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Hu, Rat
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Specificity
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This antibody recognizes the second extracellular loop of the dopamine transporter (DAT-ECD) in rats and humans.
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Tested applications
(see key)
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ICC, IHC-Fr, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC: Use at a concentration of 1 - 1.5 µg/ml. (see Hersch et al).
IHC-Fr: 1/500 (see Ma et al).
WB: 1/500. Predicted molecular weight: 74 kDa. (see Hersch et al).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane; Multi pass membrane protein.
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Research areas
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Neuroscience >> Neurotransmitter >> Transporters >> Dopamine
Neuroscience >> Neurotransmitter >> Biogenic Amines >> Dopamine
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Relevance
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Plasmalemmal neurotransmitter transporters sequester synaptic and peri synaptic transmitter into presynaptic elements. The Dopamine Transporter (DAT) is responsible for the reaccumulation of dopamine after it has been released. Levels of DAT protein expression are altered by chronic drug administration. The activity of the DAT reuptake carrier is sodium dependent, and it is suspected to play a role in such neurologic and psychiatric disorders as Parkinson's disease, Tourette's disease, schizophrenia, and addiction. It is a 12 transmembrane domain transporter with the N and C terminal regions located within the cytoplasm.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rat
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Clonality
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Monoclonal
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Clone number
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hDAT-LOOP
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Isotype
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IgG
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Purity
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Protein A purified
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Storage buffer
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Preservative: None
Constituents: PBS
Sterile Filtered
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Form
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Liquid
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Concentration
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5.800 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Dopamine Transporter antibody [hDAT-LOOP] is on this datasheet. But please do contact us if you would like any reassurance! |
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References for Dopamine Transporter antibody [hDAT-LOOP] (ab5981)
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This clone has been used in: (two most recent references)
Ma SY et al. Dopamine transporter-immunoreactive neurons decrease with age in the human substantia nigra. J Comp Neurol 409:25-37 (1999). PubMed: 10363709
Hersch SM et al. Subcellular localization and molecular topology of the dopamine transporter in the striatum and substantia nigra. J Comp Neurol 388:211-27 (1997). PubMed: 9368838
If you publish research using ab5981 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to Dopamine Transporter
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