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Products:Neuroscience >> Neurotransmitter >> Biogenic Amines >> Dopamine
Overview
Product name
Anti-Dopamine Transporter antibody [hDAT-LOOP]
See all Dopamine Transporter products (6) ...
Description
Rat monoclonal [hDAT-LOOP] to Dopamine Transporter
Specificity
This antibody recognizes the second extracellular loop of the dopamine transporter (DAT-ECD) in rats and humans.
Tested applications
IHC-FoFr, ICC, WB, IHC-Frmore details
Cross reactivity
Reacts with
Rat, Human
Immunogen
Fusion protein consisting of the second extracellular loop (amino acids 180-218).
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Sterile Filtered
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Clone number
hDAT-LOOP
Isotype
IgG
Research areas
Neuroscience >> Neurotransmitter >> Transporters >> Dopamine
Neuroscience >> Neurotransmitter >> Biogenic Amines >> Dopamine
Applications
Show applications key
Our Abpromise guarantee covers the use of ab5981 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: Use at an assay dependent concentration. (PubMed: 20207227)
ICC: Use a concentration of 1 - 1.5 µg/ml.
WB: 1/500Predicted molecular weight: 74 kDa.
IHC-Fr
IHC-Fr: 1/500IHC-Fr: 1/500
Target
Function
Amine transporter. Terminates the action of dopamine by its high affinity sodium-dependent reuptake into presynaptic terminals.
Involvement in disease
Defects in SLC6A3 are the cause of dystonia-parkinsonism infantile (DYTPRI) [MIM:613135]. It is a neurodegenerative disorder characterized by infantile onset of parkinsonism and dystonia. Other neurologic features include global developmental delay, bradikinesia and pyramidal tract signs.
Sequence similarities
Belongs to the sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family. SLC6A3 subfamily.
Cellular localization
Membrane.
Target information above from: UniProt accessionQ01959
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DA transporter antibody
- DAT 1 antibody
- DAT antibody
see all
Database links
- Entrez Gene: 6531 Human
- Entrez Gene: 24898 Rat
- Omim: 126455 Human
- SwissProt: Q01959 Human
- SwissProt: P23977 Rat
- Unigene: 406 Human
- Unigene: 10093 Rat
References for Anti-Dopamine Transporter antibody [hDAT-LOOP] (ab5981)
This product has been referenced in:
- Martínez-Cerdeño Vet al. Embryonic MGE precursor cells grafted into adult rat striatum integrate and ameliorate motor symptoms in 6-OHDA-lesioned rats. Cell Stem Cell 6:238-50 (2010). IHC-FoFr; Rat.Read more (PubMed: 20207227) »
- Ma SYet al. Dopamine transporter-immunoreactive neurons decrease with age in the human substantia nigra. J Comp Neurol 409:25-37 (1999).Read more (PubMed: 10363709) »
See all 3 publications for this product
Publishing research using ab5981? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"