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Anti-Dopamine beta Hydroxylase antibody [EPR4414] (ab109112)

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Overview

Product name

Anti-Dopamine beta Hydroxylase antibody [EPR4414]
See all Dopamine beta Hydroxylase products (11) ...

Description

Rabbit monoclonal [EPR4414] to Dopamine beta Hydroxylase

Tested applications

Flow Cyt, WB, IHC-Pmore details

Cross reactivity

Reacts with

Human

Does not react with

Mouse, Rat

Immunogen

A recombinant protein fragment corresponding to residues in Human Dopamine beta-hydroxylase (UniProt P09172).

Positive control

SH-SY5Y cell lysate; adrenal gland tissue

General notes

Produced under U.S. Patent No. 5,675,063.

Properties

Form

Liquid

Storage instructions

Store at -20°C. Stable for 12 months at -20°C

Storage buffer

PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

Purity

Tissue culture supernatant

Clonality

Monoclonal

Clone number

EPR4414

Isotype

IgG1

Research areas

Metabolism >> Types of disease >> Cancer
Metabolism >> Pathways and Processes >> Endocrine metabolism >> Hormone biosynthesis
Cancer >> Cancer Metabolism >> Metabolic signaling pathway >> Hormone biosynthesis
Neuroscience >> Neurotransmitter >> Biogenic Amines >> Dopamine

  • Western blot - Dopamine beta Hydroxylase antibody [EPR4414] (ab109112)Western blot - Dopamine beta Hydroxylase antibody [EPR4414] (ab109112) image (enlarge)

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR4414] (ab109112)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR4414] (ab109112) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab109112 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Flow Cyt

     Flow Cyt: 1/10 - 1/100.

    Flow Cyt: 1/10 - 1/100.

  • ShowHide1 Image

    WB

     WB: 1/1000 - 1/10000. De...Read more →

    WB: 1/1000 - 1/10000. Detects a band of approximately 70-75 kDa (predicted molecular weight: 69 kDa).

  • ShowHide1 Image

    IHC-P

     IHC-P: 1/100 - 1/250. An...Read more →

    IHC-P: 1/100 - 1/250. Antigen retrieval is recommended.

Application notes

Is unsuitable for ,ICC/IF or IP.

Target

Function

Conversion of dopamine to noradrenaline.

Pathway

Catecholamine biosynthesis; (R)-noradrenaline biosynthesis; (R)-noradrenaline from dopamine: step 1/1.

Involvement in disease

Defects in DBH are the cause of dopamine beta-hydroxylase deficiency (DBH deficiency) [MIM:223360]; also known as norepinephrine deficiency or noradrenaline deficiency. This disorder is characterized by profound deficits in autonomic and cardiovascular function, but apparently only subtle signs, if any, of central nervous system dysfunction.

Sequence similarities

Belongs to the copper type II ascorbate-dependent monooxygenase family.
Contains 1 DOMON domain.

Cellular localization

Cytoplasmic vesicle > secretory vesicle lumen. Cytoplasmic vesicle > secretory vesicle > chromaffin granule lumen and Cytoplasmic vesicle > secretory vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane.

Target information above from: UniProt accessionP09172 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • dbh antibody
  • DBM antibody
  • Dopamine beta monooxygenase antibody
  • Dopamine beta-hydroxylase (dopamine beta-monooxygenase) antibody
  • Dopamine beta-monooxygenase antibody
  • DOPO_HUMAN antibody
  • OTTHUMP00000022501 antibody
  • Soluble dopamine beta-hydroxylase antibody
see all

Database links

Anti-Dopamine beta Hydroxylase antibody [EPR4414] images:

  Western blot - Dopamine beta Hydroxylase antibody [EPR4414] (ab109112)

Western blot - Dopamine beta Hydroxylase antibody [EPR4414] (ab109112)

Anti-Dopamine beta Hydroxylase antibody [EPR4414] (ab109112) at 1/1000 dilution + SH-SY5Y cell lysate at 10 µg

Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size : 69 kDa
Observed band size : 70-75 kDa (why is the actual band size different from the predicted?)

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR4414] (ab109112)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Dopamine beta Hydroxylase antibody [EPR4414] (ab109112)

Immunohistochemical analysis of Dopamine beta Hydroxylase in paraffin embedded adrenal gland tissue using ab109112 at a 1/100 dilution.

References for Anti-Dopamine beta Hydroxylase antibody [EPR4414] (ab109112)

ab109112 has not yet been referenced specifically in any publications.

Publishing research using ab109112? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"