AbcamBiochemicals

Dopamine hydrochloride (ab120565)

Overview

  • Product nameDopamine hydrochloride
  • Description
    Endogenous neurotransmitter
  • Biological descriptionEndogenous neurotransmitter. Dopamine D1-5 receptor agonist. Norepinephrine and epinephrine precursor. Involved in numerous neurological functions and also in various conditions such as Parkinson's disease and schizophrenia.
  • Purity> 99%

Properties

    Applications

    Our Abpromise guarantee covers the use of ab120565 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Functional Studies Use at an assay dependent concentration.

    Dopamine hydrochloride images

    • ab32088 staining MEK1 (phospho S218 + S222) in SKNSH cells treated with dopamine hydrochloride (ab120565), by ICC/IF. Increase in MEK1 (phospho S218 + S222) expression correlates with increased concentration of dopamine hydrochloride, as described in literature.
      The cells were incubated at 37°C for 24h in media containing different concentrations of ab120565 (dopamine hydrochloride) in DMSO, fixed with 100% methanol for 5 minutes at -20°C and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab32088 (1/100 dilution) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 goat anti-rabbit polyclonal antibody (ab96899) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.

    References for Dopamine hydrochloride (ab120565)

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