Anti-Doublecortin (phospho S28) antibody - Neuronal Marker (ab23544)

Western blot - Doublecortin (phospho S28) antibody - Neuronal Marker (ab23544)

All lanes : Anti-Doublecortin (phospho S28) antibody - Neuronal Marker (ab23544) at 1 µg/ml

Lane 1 : Mouse Adult Brain Whole Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)
Lane 3 : Mouse Adult Brain Whole Tissue Lysate with Doublecortin peptide - phospho S28 (ab31511) at 1 µg/ml
Lane 4 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) with Doublecortin peptide - phospho S28 (ab31511) at 1 µg/ml
Lane 5 : Mouse Adult Brain Whole Tissue Lysate with Doublecortin peptide (ab31512) at 1 µg/ml
Lane 6 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) with Doublecortin peptide (ab31512) at 1 µg/ml

Lysates/proteins at 20 µg per lane.

Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 50 kDa
Observed band size : 50 kDa
Additional bands at : 35 kDa (possible degradation product).

Immunohistochemistry - Free Floating - Doublecortin (phospho S28) antibody - Neuronal Marker (ab23544)

Immunofluorescent staining for Doublecortin (phospho S28) in the rat habenular nucleus using Rabbit polyclonal to Doublecortin (phospho S28). The staining pattern observed with ab23544 appears to be specific, however, the staining is not very strong or contrasted - this might be expected for detection of a modification protein in unstimulated tissue. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in free floating IHC. Primary antibody ab123544 was incubated overnight at 1/1000 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.

S Pezet, Univ London Kings Coll, UK