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Products:Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Microtubules
Overview
Product name
Anti-Doublecortin (phospho S28) antibody - Neuronal Marker
See all Doublecortin products (10) ...
Description
Rabbit polyclonal to Doublecortin (phospho S28) - Neuronal Marker
Tested applications
IHC-FoFr, WBmore details
Cross reactivity
Reacts with
Mouse, Rat
Predicted to work with
Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Mouse Doublecortin, phosphorylated at S28.
(Peptide available as ab31511.)
Positive control
Mouse Adult Brain Whole Tissue Lysate and Mouse (0-days old) Brain Whole Tissue Lysate. Rat adult brain tissue.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.016% Sodium Azide
Constituents: 0.8% BSA, 80mM Boric Acid, 60mM Sodium chloride, 4mM EDTA, 20mM Sodium borate, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
Doublecortin (DCX) undergoes a cycle of dephosphorylation and phosphorylation that regulates its binding to microtubules in growing neurites. The N-terminus serine-28 is a major site of phosphorylation for DCX; this was identified by Graham et al (2004). Single mutation of Ser-28 reduced but did not abolish phosphorylation; DCX is subject to complex multi-site phosphorylation
Clonality
Polyclonal
Isotype
IgG
Research areas
Tags & Cell Markers >> Cell Type Markers >> Neuroscience Markers >> Neuronal
Neuroscience >> Cell Type Marker >> Neuron marker >> Growth Cone
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Neuroscience >> Cell Adhesion Proteins >> Cytoskeletal Proteins >> Microtubules
Applications
Show applications key
Our Abpromise guarantee covers the use of ab23544 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-FoFr: 1/1000
WB: Use a concentration of 1 µg/mlDetects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).Can be blocked with Doublecortin peptide - phospho S28 (ab31511).
Application notes
Is unsuitable for or IHC-P.
Target
Function
Seems to be required for initial steps of neuronal dispersion and cortex lamination during cerebral cortex development. May act by competing with the putative neuronal protein kinase DCAMKL1 in binding to a target protein. May in that way participate in a signaling pathway that is crucial for neuronal interaction before and during migration, possibly as part of a calcium ion-dependent signal transduction pathway. May be part with LIS-1 of an overlapping, but distinct, signaling pathways that promote neuronal migration.
Tissue specificity
Highly expressed in neuronal cells of fetal brain (in the majority of cells of the cortical plate, intermediate zone and ventricular zone), but not expressed in other fetal tissues. In the adult, highly expressed in the brain frontal lobe, but very low expression in other regions of brain, and not detected in heart, placenta, lung, liver, skeletal muscles, kidney and pancreas.
Involvement in disease
Defects in DCX are the cause of lissencephaly X-linked type 1 (LISX1) [MIM:300067]; also called X-LIS or LIS. LISX1 is a classic lissencephaly characterized by mental retardation and seizures that are more severe in male patients. Affected boys show an abnormally thick cortex with absent or severely reduced gyri. Clinical manifestations include feeding problems, abnormal muscular tone, seizures and severe to profound psychomotor retardation. Female patients display a less severe phenotype referred to as 'doublecortex'.
Defects in DCX are the cause of subcortical band heterotopia X-linked (SBHX) [MIM:300067]; also known as double cortex or subcortical laminar heterotopia (SCLH). SBHX is a mild brain malformation of the lissencephaly spectrum. It is characterized by bilateral and symmetric plates or bands of gray matter found in the central white matter between the cortex and cerebral ventricles, cerebral convolutions usually appearing normal.
Note=A chromosomal aberration involving DCX is found in lissencephaly. Translocation t(X;2)(q22.3;p25.1).
Sequence similarities
Contains 2 doublecortin domains.
Cellular localization
Cytoplasm.
Target information above from: UniProt accessionO43602
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DBCN antibody
- DBCN antibody
- Dbct antibody
see all
Database links
- Entrez Gene: 1641 Human
- Entrez Gene: 13193 Mouse
- Entrez Gene: 84394 Rat
- Omim: 300121 Human
- SwissProt: O43602 Human
- SwissProt: O88809 Mouse
- SwissProt: Q9ESI7 Rat
- Unigene: 34780 Human
see all
Anti-Doublecortin (phospho S28) antibody - Neuronal Marker images:
Western blot - Doublecortin (phospho S28) antibody - Neuronal Marker (ab23544)
All lanes : Anti-Doublecortin (phospho S28) antibody - Neuronal Marker (ab23544) at 1 µg/ml
Lane 1 : Mouse Adult Brain Whole Tissue Lysate
Lane 2 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188)
Lane 3 : Mouse Adult Brain Whole Tissue Lysate with Doublecortin peptide - phospho S28 (ab31511) at 1 µg/ml
Lane 4 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) with Doublecortin peptide - phospho S28 (ab31511) at 1 µg/ml
Lane 5 : Mouse Adult Brain Whole Tissue Lysate with Doublecortin peptide (ab31512) at 1 µg/ml
Lane 6 : Brain (Mouse) Tissue Lysate - normal tissue, 0 days old (ab7188) with Doublecortin peptide (ab31512) at 1 µg/ml
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 50 kDa
Observed band size : 50 kDa
Additional bands at : 35 kDa (possible degradation product).
Immunohistochemistry - Free Floating - Doublecortin (phospho S28) antibody - Neuronal Marker (ab23544)
Immunofluorescent staining for Doublecortin (phospho S28) in the rat habenular nucleus using Rabbit polyclonal to Doublecortin (phospho S28). The staining pattern observed with ab23544 appears to be specific, however, the staining is not very strong or contrasted - this might be expected for detection of a modification protein in unstimulated tissue. Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in free floating IHC. Primary antibody ab123544 was incubated overnight at 1/1000 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.
S Pezet, Univ London Kings Coll, UK
References for Anti-Doublecortin (phospho S28) antibody - Neuronal Marker (ab23544)
ab23544 has not yet been referenced specifically in any publications.
Publishing research using ab23544? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"