Overview

  • Product nameAnti-Dppa2 antibody
    See all Dppa2 primary antibodies
  • Description
    Mouse polyclonal to Dppa2
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length Human Dppa2 protein (AAH18070.1)

  • Positive control
    • Jurkat cell lysate. Lysate from transfected 293T cells.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab88603 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 34 kDa.

Target

  • RelevanceDppa2 is a SAP domain protein expressed only in pluripotent cells and the developing germ line.
  • Cellular localizationNuclear
  • Database links
  • Alternative names
    • cancer/testis antigen 100 antibody
    • CT100 antibody
    • Developmental Pluripotency Associated 2 antibody
    • developmental pluripotency-associated gene 2 antibody
    • developmental pluripotency-associated protein 2 antibody
    • ECAT15-2 antibody
    • embryonic stem cell (ESC) associated transcript 15-2 antibody
    • PESCRG1 antibody
    • Pluripotent embryonic stem cell related protein 1 antibody
    • Pluripotent embryonic stem cell-related gene 1 antibody
    • Pluripotent embryonic stem cell-related gene 1 protein antibody
    see all

Anti-Dppa2 antibody images

  • Anti-Dppa2 antibody (ab88603) at 1 µg/ml + Jurkat cell lysate at 50 µg

    Predicted band size : 34 kDa
    Observed band size : 34 kDa
  • All lanes : Anti-Dppa2 antibody (ab88603) at 1 µg/ml

    Lane 1 : cell lysate from Dppa2 transfected 293T cells
    Lane 2 : cell lysate from non transfected 293T cells

    Lysates/proteins at 25 µg per lane.


    Predicted band size : 34 kDa
    Observed band size : ~40 kDa (why is the actual band size different from the predicted?)

References for Anti-Dppa2 antibody (ab88603)

ab88603 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Dako antigen retrieval solution
Sample Marmoset (common) Tissue sections (Adult testis)
Specification Adult testis
Permeabilization No
Fixative Formaldehyde
Username

Zachary Yu-Ching Lin

Verified customer

Submitted Jul 18 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"