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Product name
Anti-Dppa4 antibody
See all Dppa4 products (4) ...
Description
Rabbit polyclonal to Dppa4
Specificity
The immunogen was designed to raise an antibody against mouse Dppa4. However, the antibody does not recognise a band of the expected size of Dppa4 in the mouse cell lysate tested. This may be because Dppa4 is not expressed in sufficient amounts for detection by Western Blotting in those cells. The immunogen also contained a sequence of 12 amino acids found in human Dppa4. The antibody recognises a band of approximately the expected size of Dppa4 in human embryonic stem cell lysate. Additionally, the immuogen contains a sequence of 12 consecutive amino acids which are found in human Dppa2 and a sequence of 13 amino acids of which 11 are found in mouse Dppa2. Therefore, it cannot be ruled out that the antibody recognizes Dppa2.
Tested applications
Cross reactivity
Reacts with
Human
Does not react with
Mouse
Immunogen
Synthetic peptide derived from within residues 200 to the C-terminus of Mouse Dppa4.
(Peptide available as ab316 47.)
Positive control
Human Embryonic Stem Cell Lysate; Mouse Embryonic Stem Cell Lysate;
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.016% Sodium Azide
Constituents: 0.8% BSA, 80mM Boric Acid, 60mM Sodium chloride, 4mM EDTA, 20mM Sodium borate, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Developmental Biology >> Embryogenesis >> Embryonic stem cells >> Surface molecules
Stem Cells >> Embryonic Stem Cells >> Intracellular
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Western blot - Dppa4 antibody (ab31648)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31648 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/ml. Detects a band of approximately 36 kDa (predicted molecular weight: 33 kDa).
Target
Relevance
Little is known about the Dppa4 gene, which is from the Dppa family of developmental pluripotency associated transcription factors. Dppa4 is a target of Oct4, Sox2 and Nanog.
Cellular localization
Nuclear
Alternative names
- 2410091M23Rik antibody
- Developmental pluripotency associated 4 antibody
- Developmental pluripotency associated protein 4 antibody
see all
Database links
- Entrez Gene: 55211 Human
- SwissProt: Q7L190 Human
- Unigene: 317659 Human
Anti-Dppa4 antibody images:
Western blot - Dppa4 antibody (ab31648)
All lanes : Anti-Dppa4 antibody (ab31648) at 1 µg/ml
Lane 1 : Human Embryonic Stem Cell Lysate
Lane 2 : Mouse Embryonic Stem Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) (ab28446) at 1/10000 dilution
Predicted band size : 33 kDa
Observed band size : 36 kDa (why is the actual band size different from the predicted?)
Additional bands at : 48 kDa (possible IgG,glycosylated form,non-specific binding).
ab31648 recognized a band of approximately the predicted molecular weight (34 kDa) of Dppa4 in human, but not in mouse, embryonic stem cell lysates. The identity of the ~ 50 kDa band is not known.
References for Anti-Dppa4 antibody (ab31648)
This product has been referenced in:
- Takahashi K et al. Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131:861-72 (2007). WB; Human.Read more (PubMed: 18035408) »
See 1 publication for this product
Publishing research using ab31648? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"