Anti-Drosha antibody (ab85027)

Overview

• Product nameAnti-Drosha antibodySee all Drosha primary antibodies ...
• Description
  Rabbit polyclonal to Drosha
• Tested applicationsIHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide, corresponding to a region between residue 1050 and 1100 of human Drosha (NP_037367.3)

• Positive controlHuman Breast Carcinoma, Ovarian Carcinoma, Prostate Carcinoma tissues and Mouse Teratoma tissue.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab85027 was affinity purified using an epitope specific to Drosha immobilized on solid support.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Nuclear Signaling Pathways
  • Nuclear Receptors
  • Nuclear Pore Complex

  • Epigenetics and Nuclear Signaling
  • RNAi
  • Dicer

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • RNAi

Applications

Target

• FunctionRibonuclease III double-stranded (ds) RNA-specific endoribonuclease that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DROSHA cleaves the 3' and 5' strands of a stem-loop in pri-miRNAs (processing center 11 bp from the dsRNA-ssRNA junction) to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. Involved also in pre-rRNA processing. Cleaves double-strand RNA and does not cleave single-strand RNA. Involved in the formation of GW bodies.
• Tissue specificityUbiquitous.
• Sequence similaritiesContains 1 DRBM (double-stranded RNA-binding) domain.
  Contains 2 RNase III domains.
• DomainThe 2 RNase III domains form an intramolecular dimer where the domain 1 cuts the 3'strand while the domain 2 cleaves the 5'strand of pri-miRNAs, independently of each other.
• Cellular localizationNucleus. Nucleus > nucleolus. A fraction is translocated to the nucleolus during the S phase of the cell cycle. Localized in GW bodies (GWBs), also known as P-bodies.

Target information above from: UniProt accession Q9NRR4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 29102 Human
  • Entrez Gene: 14000 Mouse
  • Omim: 608828 Human
  • SwissProt: Q9NRR4 Human
  • SwissProt: Q5HZJ0 Mouse
  • Unigene: 97997 Human
  • Unigene: 293142 Mouse

• Alternative names
  DROSHA antibodyDrosha double stranded RNA specific endoribonuclease antibodyDrosha ribonuclease type III antibody

  Etohi2 antibodyHSA242976 antibodyNuclear RNase III Drosha antibodyp241 antibodyProtein Drosha antibodyPutative protein p241 which interacts with transcription factor Sp1 antibodyPutative ribonuclease III antibodyRANSE3L antibodyRibonuclease 3 antibodyRibonuclease III antibodyRibonuclease III nuclear antibodyRibonuclease type III nuclear antibodyRibonucleaseIII antibodyRN 3 antibodyRN3 antibodyRNase 3 antibodyRNase III antibodyRNase3 antibodyRNASE3L antibodyRNaseIII antibodyRNASEN antibodyRNC_HUMAN antibody

  see all

Anti-Drosha antibody images

• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Drosha antibody (ab85027)
  ab85027 at 1/250 dilution staining Drosha in human ovarian tumor by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Drosha antibody (ab85027)
  ab85027 at 1/250 dilution staining Drosha in mouse teratoma by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Drosha antibody (ab85027)
  ab85027 at 1/250 dilution staining Drosha in human ovarian tumor by Immunohistochemistry, Formalin-fixed, Paraffin-embedded tissue. Detection: DAB staining.
• 

  Western blot - Drosha antibody (ab85027)
  All lanes : Anti-Drosha antibody (ab85027) at 1/500 dilution
  
  Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Observed band size : 145 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 63 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 8 minutes
• 

  Immunocytochemistry/ Immunofluorescence-Drosha antibody(ab85027)
  ICC/IF image of ab85027 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab85027, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h.Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.