|
|
|
|
Immunoprecipitation - Drosophila FMR1 antibody [6A15] (ab10299)
![Immunoprecipitation - Drosophila FMR1 antibody [6A15] (ab10299)](/ps/datasheet/images/ab10299_2.jpg)
(enlarge)
|
|
|
|
|
Product Name
|
Drosophila FMR1 antibody [6A15]
|
|
Product type
|
Primary antibodies
|
|
Description
|
Mouse monoclonal [6A15] to Drosophila FMR1
|
|
Immunogen
|
His-dFMR1 fusion protein (Drosophilia melanogaster) (amino-terminal 580aa).
|
|
Reacts with
|
Dm
|
|
Does not react with
(species key)
|
Hu
|
|
Tested applications
(see key)
|
ELISA, ICC/IF, IP, WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
ELISA: Use at an assay dependent dilution.
IF: Use at an assay dependent dilution.
IP: 25 ug/mg lysate (see Abreview).
WB: Use at an assay dependent dilution. Detects a band of approximately 85 kDa (predicted molecular weight: 82 kDa).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Cellular localization
|
Cytoplasmic
|
|
Research areas
|
Chromatin and Nuclear Signaling >> Chromatin Binding Proteins >> DNA / RNA binding
Neuroscience >> Neurology process >> Neural Signal Transduction
|
|
Relevance
|
Drosophila FMR1 is a RNA-binding protein that associates with translating ribosomes and acts as a negative translational regulator of specific mRNAs. Represses translation of futsch to regulate microtubule-dependent synaptic growth and function. Part of the RNA interference (RNAi)-related apparatus; double-stranded RNA induces potent and specific gene silencing. Regulates photoreceptor structure and neurotransmission in the eye. Required for stability of the central pair of microtubules in the spermatid axoneme
Fragile X syndrome is the most common inherited form of mental retardation. It is caused by loss of FMR1 gene activity due to either lack of expression or expression of a mutant form of the protein. In mammals, FMR1 is a member of a small protein family that consists of FMR1, FXR1, and FXR2. All three members bind RNA and contain sequence motifs that are commonly found in RNA-binding proteins, including two KH domains and an RGG box.The Drosophila genome contains a single gene homologous to the FXR family. dFMR1 is subjected to transcriptional and posttranscriptional regulation during development and it homomerizes, like its human counterpart. dFMR1 profile of expression recapitulates that of the human FXR protein family: it is highly enriched in muscles, in central nervous system and in gonads. In the larval brain, anti-dFMR1 also recognizes mushroom bodies, a centre that mediates learning and memory. These features make the fly an ideal system to analyse the role of the FXR family and to identify genes in the FMRP pathway.
|
|
|
Drosophila FMR1 antibody [6A15] (ab10299) |
|
Database links
|
The links below go to external sites and will open in a new browser window Entrez Gene | | 37528 (Fruit fly (Drosophila melanogaster))
| SwissProt | | Q9NFU0 (Fruit fly (Drosophila melanogaster))
|
| Unigene | | 5279 (Fruit fly (Drosophila melanogaster))
|
|
|
|
Raised in
|
Mouse
|
|
Clonality
|
Monoclonal
|
|
Clone number
|
6A15
|
|
Myeloma
|
Sp2/0
|
|
Isotype
|
IgG1
|
|
Purity
|
Protein A purified
|
|
Storage buffer
|
Preservative: 0.1% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Purification notes
|
Protein A purified from tissue culture supernatant.
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this Drosophila FMR1 antibody [6A15] is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for Drosophila FMR1 antibody [6A15] images, references, products related to ab10299 and other tools.
|
| |
Drosophila FMR1 antibody [6A15] images:
|
 Immunoprecipitation - Drosophila FMR1 antibody [6A15] (ab10299)
ab10299 used to Western blot specific immunoprecipitates.
In this experiment tissue lysate from Drosophila cellularizing embryos were immunoprecipitated with either ab10299 or and anti-FLAG antibody (at 25ug/mg lysate). Supernatant and pellet from these immunoprecipitates were then Western blotted with ab10299. The image clearly shows that ab10299 brings down dFMRP in the immunoprecipitation pellet whilst the anti-FLAG monoclonal does not. This image is courtesy of an Abreview submitted by Dr Ophelia Papoulas
See Abreview
|
| |
References for Drosophila FMR1 antibody [6A15] (ab10299)
|
|
This product has been used in: (two most recent references)
Kwak JE et al. GLD2 poly(A) polymerase is required for long-term memory. Proc Natl Acad Sci U S A 105:14644-9 (2008). Fruit fly (Drosophila melanogaster). PubMed: 18780789
Monzo K et al. Fragile X mental retardation protein controls trailer hitch expression and cleavage furrow formation in Drosophila embryos. Proc Natl Acad Sci U S A 103:18160-5 (2006). IP. PubMed: 17110444
If you publish research using ab10299 please let us know so that we can cite the reference on this datasheet.
|
| |
|
Search PubMed (MEDLINE) for references to Drosophila FMR1
|
| |
Drosophila FMR1 antibody [6A15] - more information
|
Customer reviews (feedback) regarding Drosophila FMR1 antibody [6A15] |
Customer FAQs regarding Drosophila FMR1 antibody [6A15] |
Protocols for Drosophila FMR1 antibody [6A15] |
Price and availability of products related to Drosophila FMR1 antibody [6A15] |
| |
Drosophila FMR1 antibody [6A15] - related products:
|
|
|
| |
Drosophila FMR1 antibody [6A15] - other tools:
|
Contact Abcam with a Technical Enquiry about Drosophila FMR1 antibody [6A15] |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunoprecipitation - Drosophila FMR1 antibody [6A15] (ab10299)](/index.html?datasheet=10299#ab10299_2.jpg)
