Anti-Dscam antibody (ab43847)

Overview

• Product nameAnti-Dscam antibodySee all Dscam primary antibodies ...
• Description
  Rabbit polyclonal to Dscam
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Fruit fly (Drosophila melanogaster)
  
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1150 - 1250 of Fruit fly (Drosophila melanogaster) Dscam.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab43846.)

• Positive controlThis antibody gave a positive signal in Drosophila S2 cell lysate.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Neuroscience
  • Neurology process
  • Growth and Development
  • Axonal Guidance Proteins

Applications

Target

• FunctionCell adhesion molecule that plays a role in neuronal self-avoidance. Promotes repulsion between specific neuronal processes of either the same cell or the same subtype of cells. Mediates within retinal amacrine and ganglion cell subtypes both isoneuronal self-avoidance for creating an orderly dendritic arborization and heteroneuronal self-avoidance to maintain the mosaic spacing between amacrine and ganglion cell bodies. Receptor for netrin required for axon guidance independently of and in collaboration with the receptor DCC. In spinal chord development plays a role in guiding commissural axons projection and pathfinding across the ventral midline to reach the floor plate upon ligand binding. Enhances netrin-induced phosphorylation of PAK1 and FYN. Mediates intracellular signaling by stimulating the activation of MAPK8 and MAP kinase p38.
• Tissue specificityPrimarily expressed in brain.
• Sequence similaritiesContains 6 fibronectin type-III domains.
  Contains 10 Ig-like C2-type (immunoglobulin-like) domains.
• DomainIg-like C2-type domains 7 to 9 are sufficient for interaction with NTN1 and commissural axon outgrowth. The transmembrane domain is necessary for interaction with DCC.
• Post-translational
  modificationsPhosphorylated at tyrosine residues. Phosphorylation is enhanced by netrin.
• Cellular localizationSecreted and Cell membrane. Localized in the soma, cell membrane, axon and growth cone of dissociated commissural axons.

Target information above from: UniProt accession O60469 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • GenBank: AF260530 Fruit fly (Drosophila melanogaster)
  • SwissProt: Q9NBA1 Fruit fly (Drosophila melanogaster)
  • SwissProt: Q8IGA5 Fruit fly (Drosophila melanogaster)
  • SwissProt: Q95S10 Fruit fly (Drosophila melanogaster)
  • SwissProt: Q95TG0 Fruit fly (Drosophila melanogaster)

• Alternative names
  CHD2 42 antibodyCHD2 52 antibodyCHD2 antibody

  CHD2-52 antibodyDown syndrome cell adhesion molecule antibodyDSCAM antibodyDSCAM_HUMAN antibodyhuman CHD2-52 down syndrome cell adhesion molecule 2, 9 antibodyOTTHUMP00000109193 2 antibodySD11109p antibody

  see all

Anti-Dscam antibody images

• 

  Western blot - Anti-Dscam antibody (ab43847)
  Anti-Dscam antibody (ab43847) at 1 µg/ml + Drosophila S2 Cells at 10 µg
  
  Secondary
  Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 222 kDa
  Observed band size : 235 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 1 minute